Proteinase I, an enzyme previously shown to be able to degrade contractile and cytoskeletal elements of white-croaker (Micropogon opercularis) myofibrils, was purified to apparent homogeneity by chromatography on DEAE-Sephacel, octyl-Sepharose CL 4B and arginine-Sepharose 4B. Its Mr was determined to be 269,000 by Sephacryl S-300 gel filtration. Under denaturing conditions, the enzyme dissociat...

Radix Isatidis is an official herbal medicine for treatment of infection and inflammation in China. In this study, a novel heteropolysaccharide (RIWP) was isolated from R. Isatidis through DEAE Sepharose Fast Flow column and Sepharose CL-6B column. RIWP had a molecular weight of 57 kDa and was mainly composed of glucose, galactose and arabinose with a relative molar ratio of 2.0:1.1:1.0. The cy...

p-Hydroxyacetophenone was coupled to epoxy-activated Sepharose 6B to generate an afflnity chromatographic matrix to purify aldehyde dehydrogenase. Purified beef liver mitochondrial aldehyde dehydrogenase specifically bound to the support and could be eluted with phydroxyacetophenone. A post-ammonium sulfate (3045%) fraction of bovine liver was applied to the affinity gel column and aldehyde deh...

background: factor vii concentrates are used in patients with congenital or acquired factor vii deficiency or treatment of hemophilia patients with inhibitors. in this research, immunoaffinity chromatography was used to purify factor vii from prothrombin complex (prothrombin-proconvertin-stuart factor-antihemophilic factor b or ppsb) which contains coagulation factors ii, vii, ix and x. the aim...

Thermal stability is one of the essential parameters characterizing biocatalysts with potential applications in chemical and pharmaceutical industries. Therefore, it extremely important to develop standardized procedures for enzyme studies. The paper attempts assess thermal immobilized lipases aqueous buffers: lipase B from Candida antarctica (CALB) rugosa (CRL-OF) on Octyl-Sepharose CL-4B carr...

The isolated vascularly perfused rat intestine exhibits an obligatory need for a protein carrier in order to absorb zinc. Therefore this system is ideal for use as a model to identify the plasma carrier during zinc absorption. Affinity chromatography on Blue Sepharose CL-6B was employed to separate the major serum zinc-binding proteins in the portal effluent of the perfused intestine. It was f...

We produced two monoclonal hybridoma cell lines that secrete IgG immunoglobulins with high affinities (Kd = 7.3 to 8.0 X 10(-11) mol/L, Ka = 1.25 to 1.37 X 10(10) L/mol) for 125I-labeled human prostatic acid phosphatase (PAP), and that specifically bind this enzyme from human serum. The antibodies were produced in high titers in murine ascitic fluid (700 mg/L) and in cell-culture media (30 mg/L...

Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) va...