Commercial serologic assays accurately detect adult Helicobacter pylori infection. Their use in children remains controversial. An ELISA to detect H. pylori IgG in children was developed and compared with three commercial assays. ELISA standardization was done with sera from all ages and validation was done with another cohort of sera with known H. pylori status. Three commercial serologic assa...

By Susis W. FONG, ARLENE NUCKTON AND H. H. FUDENBERG M ANY SEROLOGIC tests have been advocated as aids for the antenatal diagnosis of ABO hemolytic disease of the newborn (ABO HDN). The majority of the tests currently used in most hospital clinical laboratories for this purpose make use of certain serologic properties that differentiate the isoagglutinins into two groups. Although both groups p...

BACKGROUND The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard. METHODS The sensitivity and specificity of 5 serologic tests for S....

The effects of temperature on the results obtained in the older serological tests for syphilis employing lipoidal antigens is well known. In fact, the differences in test results obtained when certain tests were performed at different temperatures served not only as the basis for attempted differentiation of syphilitic from so-called biologic false positive reactions (Kahn, 1940), but also for ...

BACKGROUND Tests to determine serum antibody levels-the 2-tier sonicate immunoglobulin M (IgM) and immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and Western blot method or the IgG of the variable major protein-like sequence-expressed (VlsE) sixth invariant region (C6) peptide ELISA method-are the major tests available for support of the diagnosis of Lyme disease. However, the...

OBJECTIVES Celiac disease is one of the malabsorption syndromes leads to growth and development retardation in children. There is no test lonely can definitely diagnose celiac; however, the collection of clinical findings, serologic tests, intestinal biopsy, and response to treatment may diagnose it. Although diagnostic value is variable in different studies, they are used a non-invasive and ap...

The emergence of direct-acting antiviral (DAA) therapies and noninvasive measures of liver fibrosis has streamlined the management of patients with chronic hepatitis C virus (HCV) infection. DAA therapy is associated with a significantly higher rate of sustained virologic response (SVR) compared to interferon-based therapies. Concomitantly, validated noninvasive measures of fibrosis allow evalu...

OBJECTIVES. The purpose of this work was to evaluate the use of serologic testing as a screening test for inflammatory bowel disease compared with erythrocyte sedimentation rate and hemoglobin in a referred patient population with suspected inflammatory bowel disease. PATIENTS AND METHODS.A retrospective study was performed, reviewing medical charts of patients who had inflammatory bowel diseas...

Chikungunya virus (CHIKV) is present or emerging in dengue virus-endemic areas. Infections caused by these viruses share some common signs/symptoms, but prognosis, patient care, and persistent symptoms differ. Thus, accurate diagnostic methods are essential for differentiating the infections. We evaluated 4 CHIKV serologic diagnostic tests, 2 of which showed poor sensitivity and specificity.