BACKGROUND Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment....

Twenty agents with adenovirus morphology were recovered from New Zealand domestic hens by means of chick kidney cell cultures. All the agents gave distinct cytopathic effects (CPE) in cell cultures, with two different types of CPE observed. On the basis of neutralization tests, the twenty agents were assigned to four distinct serological groups. Physicochemical tests on a "prototype" strain fro...

Actinobacillus pleuropneumoniae is a primary bacterial pathogen causing swine respiratory disease and has been described in most major swine-producing regions of the world. Preliminary diagnosis of A. pleuropneumoniae is based on clinical signs and gross pneumonic lesions. Isolating and identifying the causative bacteria confirms the diagnosis. Twelve serotypes of A. pleuropneumoniae and a numb...

INTRODUCTION Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility...

BACKGROUND Detection of Plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. Whereas P. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. Given this restriction, the present study investigated whether recombinant P. vivax merozoite surface protein 1 (PvMSP-1 42 ...

detection of plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. whereas p. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. given this restriction, the present study investigated whether recombinant p. vivax merozoite surface protein 1 (pvmsp-1 42 kda) could ...

Undiagnosed and mismanaged tuberculosis (TB) continues to fuel the global TB epidemic. Rapid, accurate and early diagnosis of TB is therefore a priority to improve TB case detection and interrupt transmission. Although considerable improvements have been made in TB diagnostics, there are two major gaps in the existing diagnostics pipeline: (1) lack of a simple accurate point-of-care test that c...

Serological and non-serological tests were performed in matched samples of cerebrospinalfluidand serum from236 syphilitic patients. An increased lgG or lgM index, or both, was found about70 times more often in symptomatic neurosyphilis than in latent syphilis without involvement of the central nervous system. An increased Ig index, together with a cell count >5/4., was only found in symptomatic...

Transposition of diagnostic tests used in domestic livestock species to free-ranging and captive wildlife species has two problems. First, most existing tests have not been adequately validated in domestic livestock. Second, assumptions that a serological test will perform identically in wildlife and livestock species may not be correct, due to differences in pathogenic strains and serovars, ho...