HBL100 is a human breast epithelial cell line which has been immortalized by the defective genome of SV40 but which is not tumorigenic in nude mice. Growth experiments in defined serum-free and protein-free media revealed its ability to grow without exogenous supply of serum growth factors. Addition of heparin to the serum-free medium increased its growth rate, suggesting the release of heparin...

This study was carried out to determine the effects of supplementation of the maturation media with insulin on in vitro maturation and fertilization of bovine oocytes. In Experiment 1, cumulus-intact bovine oocytes were cultured in a maturation medium (TCM-199 containing 10% fetal calf serum, 0.02 U/ml follicular stimulating hormone and 1 microgram/ml estradiol-17 beta) with or without insulin ...

Diiron enneacarbonyl, colloidal iron particles used for removing monocytes, enhanced the 3H-thymidine uptake into the mitogen-stimulated human lymphocytes in serum-free medium, whereas neither silica particles, latex particles, carbonyl iron powder nor iron pentacarbonyl did. Because monocytes had no influence on the enhancing effect or diiron enneacarbonyl did not bind to PHA, it was suggested...

Dear Editor: A number of laboratories and companies have reported on the development of serum-free media for the growth of myelomas and hybridomas in tissue culture (1-4,6-9). Due to the different growth requirements of different cell lines, these serum-free media generally have not been broadly applicable. For example, HB101 (Hana Biologies) supported the growth of SP2/0 cells, SP2/O-derived h...

Protocols for primary cultures of teleost cells are commonly only moderately adjusted from similar protocols for mammalian cells, the main adjustment often being of temperature. Because aquatic habitats are in general colder than mammalian body temperatures and teleosts have gills in direct contact with water, pH and buffer capacity of blood and extracellular fluid are different in fish and mam...

In this study a scaffoldless approach was employed with two different cell sources and biochemical stimuli to engineer a spectrum of fibrocartilages representative of the different regions of the knee meniscus. Constructs composed of 100% fibrochondrocytes (FC) or a 50:50 co-culture of fibrochondrocytes and chondrocytes (CC) were cultured in 10% fetal bovine serum medium or serum-free "chondrog...

To examine whether a human colony-stimulating factor (CSF)producing cell line, T3M-1, can propagate and secrete CSF without protein supplements, long-term cultivation of the cells was carried out in a protein-free chemically defined medium. By the use of stepwise decreases in the fetal bovine serum concen tration, continuous growth of T3M-1 cells has been established in a protein-free F-10 medi...

To examine whether a human colony-stimulating factor (CSF)-producing cell line, T3M-1, can propagate and secrete CSF without protein supplements, long-term cultivation of the cells was carried out in a protein-free chemically defined medium. By the use of stepwise decreases in the fetal bovine serum concentration, continuous growth of T3M-1 cells has been established in a protein-free F-10 medi...

BACKGROUND It is well established that ovarian steroids modulate gonadotropin secretion from anterior pituitary cells. It has been speculated that insulin and IGF-I might influence gonadotropin secretion. OBJECTIVE To investigate the effects of IGF-I and estradiol alone, or combinations of IGF-I with insulin and estradiol on GnRH-stimulated LH release from female rat pituitary cells in serum-...