An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneratio...

Plants, unlike animals, possess a unique developmental plasticity, that allows them to adapt changing environmental conditions. A fundamental aspect of this plasticity is their ability undergo postembryonic de novo organogenesis. This requires the presence regulators trigger and mediate specific spatiotemporal changes in programs. The phytohormone cytokinin has been known as principal regulator...

Micropropagation protocol of Pimpinella candolleana an important ethno medicinal herb has been developed for the first time. High frequency plantlet regeneration was achieved from shoot tip explants of P. candolleana. Effect of growth regulators in different concentrations and combination of BAP, KN, NAA, IAA and IBA on in vitro morphogenesis was tried. The highest regeneration response was obs...

Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultur...

An efficient plant regeneration protocol has been developed from leaf explants of Stevia rebaudiana, an economically and medicinally important herbaceous plant species belonging to the family Asteraceae. Plant regeneration and multiple shoot proliferation was initiated from leaf explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) and kinetin (KN) in c...