A high-throughput sample preparation protocol based on the use of 96-well molecular weight cutoff (MWCO) filter plates was developed for shotgun proteomics of cell lysates. All sample preparation steps, including cell lysis, buffer exchange, protein denaturation, reduction, alkylation and proteolytic digestion are performed in a 96-well plate format, making the platform extremely well suited fo...

treatment protocols with low-level laser (also called ‘soft laser therapy) have been used in health care systems for more than three decades. bearing in mind the suitable sub-cellular absorption and the cellular-vascular impacts, low-level laser may be a treatment of choice for soft tissues. low-level lasers have played crucial and colorful roles in performing periodontal surgeries. their anti-...

Mass spectrometry (MS) is an analytical technique which is used to measure sample’s molecular mass by measuring mass to charge ratio. In this method, a soft ionization technique like ESI (Electro Spray Ionization) is used to generate charge ions. Now days, this is the most popular method for protein identification and quantitation. In shotgun proteomics proteins are digested into smaller peptid...

AbstractGene therapy is a therapeutic approach to deliver genetic material into cells to alter their function in entire organism. One promising form of gene delivery system (DDS) is liposomes. The success of liposome-mediated gene delivery is a multifactorial issue and well-designed liposomal systems might lead to optimized gene transfection particularly in vivo. Liposomal gene delivery systems...

AbstractGene therapy is a therapeutic approach to deliver genetic material into cells to alter their function in entire organism. One promising form of gene delivery system (DDS) is liposomes. The success of liposome-mediated gene delivery is a multifactorial issue and well-designed liposomal systems might lead to optimized gene transfection particularly in vivo. Liposomal gene delivery systems...

We consider the problem of shotgun identification patterns on groups, which extends previous work DNA sequences and labeled graphs. A a group $G$ is specified by two finite subsets $C \subset G$ $K alphabet $\mathcal{A}$. In such problems, there ``global" pattern $w \in \mathcal{A}^{CK}$, one would like to be able identify this (up translation) based only observation ``local" $K$-shaped subpatt...

In this supplement, we analyze the system-level bottlenecks of Shotgun’s scalability on multicore. Currently, we are able to get maximum of about 3− 4× speedup on 8 cores. To analyze multicore performance, we run a series of tests which try to isolate effects of various factors. Our analysis indicates that Shotgun is held back by memory access, but we give suggestions for future improvements to...

In sediment-type microbial fuel cells (sMFCs) operating in rice paddy fields, rice-root exudates are converted to electricity by anode-associated rhizosphere microbes. Previous studies have shown that members of the family Geobacteraceae are enriched on the anodes of rhizosphere sMFCs. To deepen our understanding of rhizosphere microbes involved in electricity generation in sMFCs, here, we cond...