Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver-staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments cur...

A characteristic feature of apoptosis is the degradation of genomic DNA into discrete oligonucleosome fragments by the action of a Ca2+/Mg2+-dependent endonuclease. The endonuclease relevant to apoptosis has not been identified, but several possible enzymes have been described (2,4–6). One technique to identify potential nuclease activities is zymography, where protein extracts are resolved by ...

An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chro...

The endocrine cells of the gastric mucosa of Rana temporaria have been studied according to the ultrastructure, the staining properties of the granules with Masson Fontana's and Grimelius' silver methods, silver impregnation of Davenport on deplasticised semithin sections and immunocytochemical techniques. Seven different types of endocrine cells have been described. Six were regarded as belong...

A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used f...