To transfer only part of genome, X-ray irradiated suspension cell protoplasts of N. Ian gsdorfii were fused with suspension cell protoplasts of N. glauca by polyethylene glycol. Somatic hybrid calli were selected by the growth in the hormone-free medium. Some of somatic hybrid calli from fusion with irradiated protoplasts indicated the loss of small subunit polypeptide of fraction 1 protein whi...

Conservation of rare species is essential for maintaining ecosystem function. Quercus hondae a evergreen oak (Cyclobalanopsis) endemic to Japan. This found in several locations Southwestern Japan; small populations remain the tutelary forests Japanese shrine. To evaluate genetic diversity and phylogeographic structure this species, 11 microsatellite loci chloroplast DNA sequences are analyzed 1...

BACKGROUND The introduction of pharmaceutical traits in tobacco for commercial production could benefit from the utilization of a transgene bioconfinement system. It has been observed that interspecific F1Nicotiana hybrids (Nicotiana tabacum × Nicotiana glauca) are sterile and thus proposed that hybrids could be suitable bioconfined hosts for biomanufacturing. We genetically tagged hybrids with...

Few cases of spontaneously horizontally transferred bacterial genes into plant genomes have been described to date. The occurrence of horizontally transferred genes from the T-DNA of Agrobacterium rhizogenes into the plant genome has been reported in the genus Nicotiana and in the species Linaria vulgaris. Here we compare patterns of evolution in one of these genes (a gene encoding mikimopine s...

Being an energy source of another origin, the compression ignition (CI) engine’s typical design parameters might not suit Simarouba oil methyl ester (SuOME). Present experimental investigation targets are determining effects engine parameters, including fuel injection pressure and nozzle geometry, on engine, concerning performance emissions such as carbon monoxide (CO), unburnt hydrocarbon (HC)...