Successful delivery of irf5-siRNA by PEI-LBL-NCs resulted macrophages phenotype change from the M1 to M2 and accelerated wound healing.

Magnetic measurements on a high-T, superconductor YBa2Cu30q are reported. Diamagnetism sets in, at low fields, below a temperature T=90 K. At low T the field-cooled magnetization becomes positive again. We show that the low-T phase is still superconducting and the apparent reentry behavior is due to strong paramagnetic contributions. Glassy features appear below a temperature whose field depend...

SUMMARY To facilitate the designing process for vector-based siRNA and siRNA cassette, a tool set has been developed consisting of a siRNA target finder, a siRNA construct builder and a siRNA sequence scrambler. The siRNA target finder is used to identify candidate siRNA target sites. The program automates homology filtering, minimizes non-specific cross-reaction, filters target sites based on ...

Objective: To Research of hiperfect-mediated RNA interference targeting human telomerase reverse transcriptase gene (hTERT) on the biological behavior malignant melanoma cell line A375.Methods: Specific siRNAs with green fluorescent labeling different concentrations were transfected into A375 cells by using transfection reagent (Hiperfect) at volumes . The efficiency was determined fluorescen m...

Macrophages play a key role in the development of many diseases, like tissue injury, cancer and autoimmune diseases. So far, single-drug loaded nanoparticles have been developed to target macrophages. Nevertheless, macrophage dysregulation can induce multiple conditions, i.e., inflammation fibrosis. Therefore, simultaneous co-delivery small molecule drug interfering RNA (siRNA) for gene silenci...

RNA interference (RNAi) is the process by which short-interfering RNA (siRNA) target a specific mRNA for degradation through interactions with an RNA-induced silencing complex (RISC). Here, a clear correlation between siRNA localization, cellular uptake, and RNAi activity was discovered by delivering siRNA into cells using siRNA-TAT(47-57) peptide, siRNA-TAT(47-57)-derived oligocarbamate conjug...