Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.

Ten years ago, intermediate reaction steps in the catalytic cycle of cytochrome c oxidase were titrated with phosphorylation potential in isolated mitochondria, and the results were interpreted as evidence for thermodynamic linkage of proton translocation exclusively to the oxidative reaction steps of the catalytic cycle [Wikström, M. (1989) Nature 338, 776-778]. Michel has recently argued that...

although there is enough knowledge indicating on the influence of frequency content of input motion on the deformation demand of structures, state-of-the-practice seismic studies use the intensity measures such as peak ground acceleration (pga) which are not frequency dependent. the v max/a max ratio of strong ground motions can be used in seismic hazard studies as the representative of frequen...

evidence acquisition a systematic literature searching of databases medline, embase, and google scholar was done from 1982 and updated in december 2015. search terms were “cholecystectomy”, “laparoscopic cholecystectomy”, “four port laparoscopic cholecystectomy”, “single-incision laparoscopic surgery”. context laparoscopic approach with its several techniques has become the standard method for ...