We hypothesised that normal skeletal muscle stimulated intensely either in vitro or in situ would exhibit reactive oxygen species (ROS)-mediated contractile apparatus changes common to many pathophysiological conditions. Isolated soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat were bubbled with 95% O(2) and stimulated in vitro at 31°C to give isometric tetani (50 Hz for 0.5 ...

New biodegradable multiblock amphiphilic and thermosensitive poly(ether ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] (PHB), poly(ethylene glycol) (PEG), and poly(propylene glycol) (PPG) blocks were synthesized, and their aqueous solutions were found to undergo a reversible sol-gel transition upon temperature change at very low copolymer concentrations. The multiblock poly(ether es...

CONTEXT Solubility limitation of BCS class II drugs pose challenges to in vitro release. OBJECTIVE To investigate the miscibility of Aprepitant (APR) and Soluplus(®) (SOL) for hot melt extrusion (HME) viability and improved in vitro release of APR. METHODS Solubility parameters of APR and SOL from group contribution methods were evaluated. Heat-cool-heat differential scanning calorimetry (D...

Tissue loss or organ failure caused by injury or damage is one of the most serious and costly problems in human health care. Tissue engineering, proposed by Langer et al. in the early 1990’s (Langer & Vacanti, 1993), is an emerging strategy of regenerative biomedicine that holds promise for the restoration of defect tissues and organs. The concept of tissue engineering is defined as “the applic...

In the past few years, an increasing number of in situ-forming systems have been reported in the literature for various biomedical applications, including drug delivery, cell encapsulation, and tissue repair. There are several possible mechanisms that lead to in situ gel formation: solvent exchange, UV-irradiation, ionic cross-linkage, pH change, and temperature modulation. The thermosensitive ...

PURPOSE An in situ forming hydrogel was evaluated as a potential vitreous substitute in rabbits. METHODS The hydrogel used a disulfide cross-linker that was then reduced to produce an injectable thiol-containing polymer solution. The disulfide cross-links reformed by air oxidation of the thiols and produced a stable hydrogel once inside the eye. The polymer was clear, autoclavable, and could ...

Article history: Received on: 29/05/2014 Revised on: 12/06/2014 Accepted on: 30/06/2014 Available online: 28/07/2014 In this study effect of gamma irradiation sterilization and storage conditions on thermal properties of phase sensitive granisetron in situ forming implant (ISFI) was investigated. ISFI was prepared by mixing Poly (DLlactide-co-glycolide) (PLGA) with dimethylsulfoxide (DMSO) : pr...

Stress development in thin layers of lead titanate prepared by sol-gel processing was monitored by in situ laser reflectance measurements. Layers were spin coated onto silicon substrates and thermally cycled to 500 °C. The shrinkage normal to the rigid substrate was determined by in situ ellipsometry. Changes that occurred on drying and firing, which related to densification and stress developm...

In this study, an ion-activated ketotifen ophthalmic delivery system was developed by using a natural polysaccharide, deacetylase gellan gum. Its rheological characteristics, stability, in vitro gelation, release in vitro, and pharmacodynamic activity in vivo were investigated. The formulation had an optimum viscosity that will allow easy drop as a liquid, which then underwent a rapid sol-gel t...