Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830...

Tests of 29 human variable number of tandem repeat probes in inbred mouse lines showed that 80% (23/29) cross-hybridize, and 48% (14/29) produce multiple band, minisatellite polymorphisms (fingerprint patterns). Mini-satellite-type polymorphisms detected by 11 probes were characterized in eight different strains; on average, 240 polymorphic differences were detected between pairs of strains. Re...

Using 20 pairs of SSR primers situated on each chromosome the maize genetic makeup, 27 drought-tolerant genotypes obtained from Germplasm Unit International Institute Tropical Agriculture (IITA), Ibadan, Nigeria, were examined for diversity. The samples' genomic DNA (gDNA) was extracted using CTAB procedure specified by CIMMYT (2005). All markers used in this investigation extremely polymorphic...

mahaleb (prunus mahalab l.) is used as a rootstock for cherries in iran and many other countries. in this research, rapd markers were used to determine the genetic diversity among eight genotypes of mahaleb evaluated in two separate research grardens in karaj, iran. these genotypes are used as mother plants for seed production. totally 40 random primers were examined for pcr reactions on the te...

Random amplified polymorphic DNA fingerprinting was performed to assess the genetic diversity among rarely cultivated traditional indica rice (Oryza sativa L.) varieties collected from a tribal hamlet of Kerala State, India. A total of 664 DNA bands amplified by 15 primers exhibited 72.9% polymorphism (an average of 32.3 polymorphic bands per primer). The varieties Jeerakasala and Kalladiyaran ...

CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...

The effects of induced mutation produced by five different doses of gamma irradiation (20, 25, 30, 40, and 45 Gy) were determined using molecular approaches in Vitis vinifera cultivars, namely Thompson Seedless (Sultani Çekirdeksiz) (progenitor of seedless vinifera variety) and Kalecik Karasi (one of the best quality wine grape variety of Turkey). Mutant candidates were selected through morphol...