Ascorbate oxidase from pumpkin (Cucurbita sp.) was purified from a commercially available preparation. A single polypeptide band with M(r) 64,000 was detected after sodium dodecylsulfate-polyacrylamide gel electrophoresis of the purified enzyme. In double immunodiffusion tests, antiserum against the purified preparation formed a single precipitin line with the crude extract from pumpkin fruit t...

Activation of aerobically aged rat liver and Pseudomonas acidouorans tryptophan-2,3-dioxygenase (EC 1.13.1.12) by reducing agents was studied in an attempt to define the chemical nature of the species which achieves the reduction. With the use of two enzymes, superoxide dismutase and catalase, reductive activation by sodium ascorbate, sodium borohydride, hydroperoxide, and dithiothreitol was sh...

Objective:The objective of this in - vitro study was to evaluate the effect different antioxidants reversal deleterious bleaching on shear bond strength composite resin bleached enamel.Materials and methods: Seventy bovine incisors were used. The specimens randomly divided into seven groups (10 each) as follow: Group 1: no treatment antioxidant application. 2: only without application immediate...

The aim of this work was to obtain a black coffee brew to be consumed hot by extension of its shelf life, by addition of additives. Four pH-regulator agents (sodium and potassium carbonates and bicarbonates), one pH regulator and antioxidant (sodium citrate), three antioxidants [sodium ascorbate, ethylenediaminetetracetic acid (EDTA), and sodium sulfite], and lactoserum were tested by sensory a...

TO THE EDITOR: Research has shown that intravenous, but not oral ascorbate, can produce concentrations selectively toxic to cancer cells, which has renewed the interest in ascorbate as therapy for cancer.1,2 The protocol was approved by the ethics committee of The Center for Improvement of Human Functioning, Wichita, KS. Written informed consent was obtained. Methods. We conducted a mini-pilot ...

The effects of different levels black pepper (5, 10 or 15 g/kg) and the use ascorbate (without with ascorbate) on volatile compounds sucuk were examined in study. samples belonging to each group extracted by solid phase micro-extraction (SPME) analyzed gas chromatography-mass spectrometry (GC-MS). Ascorbate was not effective aldehydes, aromatic hydrocarbons, esters, acids terpenes. While high l...

In most southern provinces of Iran, Soil Salinity is a growing problem particularly in the irrigated agricultural areas and has been found to reduce crop yields. In order to investigate the role of pod removal in seed germination, seedling growth and antioxidant enzymes activity in annual medics (Medicago scutella L.) under salinity stress conditions, a factorial experiment was conducted at Col...

Indian mustard [Brassica juncea (L.) Czern & Coss] is one of the most important oilseed crops worldwide yet drought and salinity stress significantly reduced its growth yield. The research was carried out in order to test effect on osmoprotectants (total soluble sugars, proline), antioxidant molecules (ascorbate, ?-tocopherol) ionic content from dry sample leaves introgression lines varieti...