Polyacrylamide Gel Electrophoresis (PAGE) is both an analytical and preparative tool widely used in protein analysis. Proteins are separated by PAGE in an electric field according to their size, shape and charge both in their native configuration (native PAGE) or in the presence of denaturing agents such as the ionic detergent sodium dodecyl sulfate (SDS-PAGE). A third type of electrophoretic t...

This article aims to study the effect of concentration with additives on kinematic viscosity crude oils. Additionally, influence solvents that reduces heavy oil significance their functions in flow rate oil, which is transported by conveyor pipes long distances. Different concentrations polymers different types named as Polyvinyl chloride (PVC), Sodium dodecyl sulfate (SDS) and Carboxymethyl ce...

Cyclooxygenase-2 (COX-2) Renal mesangial cells (RMC) Wild type (WT) Adenovirus (Ad) Tumour necrosis factor α (TNFα) Endothelin-1 (ET-1) Interleukin 1β (IL-1β) Prostaglandin E2 and prostaglandin I2 (PGE2 and PGI2) Annexin V (AV) Fluorescein isothiocyanate (FITC) Propidium iodide (PI) Viable (V), early membrane intact apoptotic (EA) and necrotic (N) Hanks Buffered Saline Solution (HBBS) Phosphate...

Advanced BioMatrix offers a large number of extracellular matrix (ECM) and three dimensional (3D) scaffold products with one of the most extensive Type I collagen product offerings (see Exhibit 1). One analytical method for evaluating the quality, purity and consistency of the products is Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE electrophoresis). The purpose of the do...

A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4'-azobenzene...

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a technique that is used to separate proteins in a mixture for the purpose of identifying them individually. Proteins coated in SDS (which gives them a negative charge), are pulled through a thin gel by an electrical current. They separate on the gel based on size--the larger the protein, the slower it moves. Proteins can be stained w...

Abbreviations: CAPS: N-cyclohexyl-3-aminopropanesulfonic acid cDNA: omplementary DNA to mRNA CHES: N-cyclohexyl-2-aminoethanesulfonic acid. DTT: dithiothreitol FPLC: fast protein liquid chromatography IPTG: isopropylthio-β-D-galactoside LB: Luria-Bertani MES: 2-(N-morpholino)ethanesulfonic acid Ni-NTA: Nickel-nitriloacetic acid ORF: open reading frame PCR: polymerase chain reaction PMSF: phenyl...

The glycoprotein (GP) llb-Illa complex was isolated from human platelet membranes by immunoaffinity chromatography using a monocbonal antibody specific for GP bIb-lIla. GP llb and lIla were further separated in the presence of sodium dodecyl sulfate (SDS) by gel filtration high-performance liquid chromatography (HPLC). Two cycles of this procedure yielded almost complete separation of homogeneo...

We wish to report a powerful methodology for analysis and interpretation of complex fluorescence decays and other kinetic data, based on the addition of convolution analysis to standard (individual and global) analysis. The method was applied to cases involving excited state proton transfer of flavylium salts in water, in water-1,4-dioxane mixtures and in micellar solutions of sodium dodecyl su...

The present work describes a procedure for determining apolipoproteins (apo) B-100 and B-48 in subfractions of triglyceride-rich lipoproteins by analytical sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie staining. The chromogenicity of the two apoB species was found to be almost equal, and independent of lipoprotein particle size. Both proteins were sensitive...