Digested sludge (DS) is a waste product of anaerobic digestion that produced during the biodegradation excess sludge. It cannot be used as substrate for further biogas production owing to its recalcitrant nature. In present study, we heat treatment technique convert DABYS microflora (DABYS = digested sludge-assimilating and biogas-yielding soil microflora), which degraded DS methane gas, could ...