Evolutionary operation (EVOP) factorial design technique was explored in order to economically produce amylase and protease at their optimum level in a single bioreactor by modified solid-state fermentation. Maximum yields of amylase and protease were achieved, using wheat bran as a substrate by a highly potent, locally isolated strain of Aspergillus awamori: Nakazawa MTCC 6652. The strain had ...

A solid – liquid – gas, multiphase reactor, called Inverse Fluidized Bed Bioreactor (IFBBR) was used in this study for the treatment of starch industry wastewater of high organic content and acidic nature. Mixed culture obtained from the sludge, taken from starch industry effluent treatment plant, was used as microorganisms. Experiments were carried out in an Inverse fluidized bed bioreactor, a...

Studies were conducted to evaluate Bacillus atrophaeus spores’ production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 6...

A solid – liquid – gas, multiphase reactor, called Inverse Fluidized Bed Bioreactor (IFBBR) was used in this study for the treatment of starch industry wastewater of high organic content and acidic nature. Mixed culture obtained from the sludge, taken from starch industry effluent treatment plant, was used as microorganisms. Experiments were carried out in an Inverse fluidized bed bioreactor, a...

Major environmental concerns of municipal landfills revolve around quantity and quality of leachate, gas generation, and decomposition processes occurring therein. Minimizing the time period for maximum biodegradation to reduce leachate and gas emissions after landfill closure, ease the requirement of leachate treatment, and reclamation of landfill site is the most sought after managers proposi...

This proposal is in response to item 13 of the FCSHWM request for proposals asking for research that examines the long-term treatment and disposal options for landfill leachate. Mature landfill leachates will require a complex sequence of biological, physical, and chemical treatment processes to reach discharge limits. It is likely that ammonia-nitrogen, humics, and xenobiotic compounds in thes...

Streptomyces citreus CBS 109.60 produced geosmin and a complex pattern of other volatile compounds during cultivation in a 2.5-liter laboratory bioreactor. Volatiles were isolated from disrupted cells, from the culture medium, and from the waste air of the bioreactor by adsorption on Lewatit OC 1064MD. Quantitative and qualitative analyses were carried out using capillary gas chromatography and...

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production ...

Growing hairy roots in bioreactors has proven challenging. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. Compared to most liquid-phase bioreactors, this gasphase bioreactor offers a completely different environment for growing roots. Design, modeling, and some of the unique engineering aspects inherent to mi...

Biosurfactants are surface active substances which reduce interfacial tension and are produced or excreted at the microbial cell surface. We evaluated the biosurfactant production by Aspergillus fumigatus and Phialemonium sp. in solid state processes using fixed-bed column reactors. We evaluated two media, rice husks alone (simple support) and rice husks plus defatted rice bran (complex support...