Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...

background: escherichia coli (e. coli) is the most extensively used host for the production of recombinant proteins. however, most of the eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. reteplase as a highly disulfide-bonded recombinant protein is an example of difficult to express protein in e. coli. methods: in this s...

Analysis of circular dichroism spectra of proteins provides information about protein secondary structure. Analytical methods developed for such an analysis use structures and spectra of a set of reference proteins. The reference protein sets currently in use include soluble proteins with a wide range of secondary structures, and perform quite well in analyzing CD spectra of soluble proteins. T...

soy protein concentrate (spc) is a functional and nutritional product which is produced under conditions where soy proteins are immobilized in soy flour and soluble carbohydrates are removed. in this study, four different soy flours including defatted soy flour (fat content 3.67, pdi 55.10), defatted and toasted soy flour (fat content 3.78, pdi 10.72), low fat soy flour (fat content 14.34, pdi ...

Cataract is clouding of the eye lens that reduces the amount of incoming light and results in deteriorating vision. Blindness is thought to reach 75 million by 2020. Of these, unoperated cataract may be expected to account for at least 35 million. Thus, the burden of cataract is increasing remorselessly. Embelica officinalis is reported to have a very good antioxidant property and thus we hypot...

in order to investigate the effects of supplementary calcium chloride and potassium nitrate on plant growth of ammi (carum copticum l.) in different salinity levels and nutrient solutions (ns), an experiment based on randomized complete block design (rcbd) with factorial arrangement was conducted under greenhouse condition in the faculty of agriculture, vali-e-asr university of rafsanjan, durin...

the influence of water deprivation on potted grape (vitis vinifera l.) was investigated. leaf mass area (lma) and biochemical responses, including chlorophyll, total protein, peroxidase activity, soluble carbohydrates, photosynthesis and stomatal conductance were assessed in leaves of ‘sahani’ and ‘bidane sefid’ grape cultivars subjected to progressive drought (soil water potentials of: -0.2, -...

HIGHLIGHTS Chimeric protein P48-40 of Mycoplasma agalactiae was designed and expressed in E.coli BL21 (DE3). highly soluble form purified with yield 33mg/Lby using Nickel resin. Novel showed high performance for detection specific antibodies 100% sensitivity specificity.