Phenylacetaldehyde reductase (PAR) is suitable for the conversion of various aryl ketones and 2-alkanones to corresponding chiral alcohols. 2-Propanol acts as a substrate solvent and hydrogen donor of coupled cofactor regeneration during the conversion of substrates catalyzed by PAR. To improve the conversion efficiency in high concentrations of substrate and 2-propanol, selection of a PAR muta...

A structural polymorphism of nucleic acids, which depends on the concentration of cations and the conditions of hydration, are strongly involved with interactions between DNA and proteins. In this paper, we report that different DNA sequences bound to hyperthermostable TATA-box-binding protein (PhoTBP) at different combinations of temperature and salt concentration in in vitro selection experim...

Washing is a key step in pharmaceutical isolation to remove the unwanted crystallization solvent (mother liquor) from active ingredient (API) filter cake. This study looks at strategies for optimal wash selection, which minimizes dissolution of API product crystals while preventing precipitation or impurities. Selection solvents avoid both these phenomena can be challenging but essential mainta...

The nonsynonymous to synonymous substitution rate ratio (omega = d(N)/d(S)) provides a sensitive measure of selective pressure at the protein level, with omega values <1, =1, and >1 indicating purifying selection, neutral evolution, and diversifying selection, respectively. Maximum likelihood models of codon substitution developed recently account for variable selective pressures among amino ac...

This thesis describes efforts made on the development of an existing catalytic incinerator. The development work, called process characterization, consists of four general parts. These are the development of measurement methodology, the studying of construction materials, the selection of suitable catalysts and the testing of the effects of process operation conditions. The two application area...

In situ product recovery (ISPR), in the form of an extractive fermentation process, can increase productivity and titers sustainable production platform chemicals. To establish a guideline for development industrially relevant processes such bio-based compounds, wide screening was performed, mapping potential extensive range solvents solvent mixtures. Besides biocompatibility with Saccharomyces...

Abstract Poly-parameter Linear Free Energy Relationships (PPLFERs) based on the Abraham solvation model are a useful tool for predicting and interpreting equilibrium partitioning of solutes in solvent systems. The focus this work is neutral organic liquid solvent-air This follow-up to previous (Brown, 2021) which developed predictive empirical correlations between solute descriptors system para...