The kinetics of the degradation of the inhibitors of the nitric oxide synthesis, NG-nitro-L-arginine methyl ester and NG-nitro-L-arginine, were examined in human amniotic fluid and umbilical vein blood. The reaction rate constants were calculated or estimated using the time-controlled concentration course of both substances. These concentrations were measured by high-performance liquid chromato...

Light induction of light-harvesting chlorophyll a/b-binding protein (LHCP) mRNA accumulation was studied in light-dark synchronized cultures of Chlamydomonas reinhardi. LHCP mRNA accumulation was prevented by the chlorophyll-synthesis inhibitor alpha,alpha-dipyridyl which blocks late steps in the chlorophyll biosynthetic pathway and leads to the accumulation of the porphyrin intermediate magnes...

1. Sodium (N-acetyl-N-phenylhydroxylamine beta-d-glucosid)uronate was isolated from the urine of rabbits receiving N-acetyl-N-phenylhydroxylamine. 2. Its chemical structure was confirmed by the correspondence of the infrared spectrum of its tri-O-acetyl methyl ester derivative with the tri-O-acetyl methyl ester derivative of an authentic specimen prepared by the Koenigs-Knorr synthesis.

Studies on the exploration of alternate fuels obtained from renewable sources of energy to supplement conventional fossil fuels are being carried out throughout the world. The edible and non edible oils are being tried to either supplement or to replace diesel as fuel in CI engines. India is the net importer of edible oils and, therefore, emphasis is being laid to explore the possibility of usi...

Reaction of aryl methyl ketone with isatin in the presence of a base afforded 2- arylquinoline-4-carboxylic acid (3). Reaction of diazomethane with compound 3 yielded the ester 4. Compound 5 was prepared from the reaction of N, N'-dialkylethyl (or propyl) arnine with 4. Addition of hydrazine hydrate to compound 4a gave 2- (2- pyridyl) quinoline-4-carboxylic acid hydrazide (6). Reaction of l...

Cystinosis is an autosomal recessive disorder characterized by a high intracellular cystine concentration. To establish an in vitro model of this disorder and examine the mechanism of the proximal tubule transport defect seen with elevated intracellular cystine concentrations, rabbit proximal convoluted tubules (PCT) were perfused in vitro. PCTs were loaded with cystine using cystine dimethyl e...

In a screening for antifungal metabolites, two indole compounds of mixed biogenesis, 1H-indole-3-carboxylic acid, 1-(1,1-dimethyl-2-propenyl) methyl ester and 1H-indole-3-carboxylic acid, 1-(2,3-dihydroxy-1,1-dimethylpropyl) methyl ester were isolated from a culture of the basidiomycete Aporpiums caryae. The structural elucidation of these compounds was accomplished by spectroscopic methods.

Poly(ester-acetals) have been prepared from isopropylideneglyceryI azelaaldehydate dimethyl acetal and from methyl azelaaldehydate glycerol acetal. Acid hydrolysis of isopropylideneglyceryl azelaaldehydate led to oligomeric poly (ester-acetals) with six to seven repeating units and carboxylic acid endgroups from which the sodium salt and the methyl ester could be prepared. The polymer sodium sa...

1. The inhibition of pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanylglycine by products and product analogues was studied. 2. The non-competitive nature of the inhibition by the product N-acetyl-l-phenylalanine confirms an ordered release of products, and points to a common mechanism (involving an amino-enzyme) for pepsin-catalysed transpeptidation and hydrolysis reactions...