The methylation status in the human-muscle enolase gene (ENO3) was assayed. Previous sequence data and MspI cleavage sites indicate the presence of a 5' CpG-rich island of at least 4 kb: none of 22 characterized MspI CCGG sites is methylated in any of muscle, sperm or brain DNA. However a complex pattern of complete and partial methylation of MspI sites that is different between tissues is obse...

x > c) .3 0.6 ...c) Vanaman, T. C., Sharief, F. & Watterson, D. M. (1977) in Walsh, M. P., Vallet, B., Autric, F. & Demaille, J. G. (1979) J . Biol. Calcium-Binding Proteins and Calcium Function (Wasserman, R. H., Corradino, R. A., Carafoli, E., Kretsinger, R. H., MacLennan, D. H. & Siegal, F. L., eds.), pp. 107-116, North-Holland, Wong, P. Y. K. & Cheung, W. Y. (1979) Biochem. Biophys. Res. Am...

The article by Ravibabu, et al, 1 provides important new data in the field of biomarkers to monitor exposures to lead within the workplace, as in the lead acid battery manufacturing. They found that the serum neuron-specific enolase (NSE) levels are not elevated in association with concentrations of blood lead levels among workers in the lead acid battery manufacturing plant. In our (unpublishe...

We have cloned, over-expressed and purified enolase from Plasmodium falciparum strain NF54 in Escherichia coli in active form, as an N-terminal His6-tagged protein. The sequence of the cloned enolase from the NF54 strain is identical to that of strain 3D7 used in full genome sequencing. The recombinant enolase (r-Pfen) could be obtained in large quantities (approximately 50 mg per litre of cult...