Two validated uv spectrophotometric methods for the simultaneous estimation of Febuxostat and Diclofenac in a multicomponent dosage form have been developed, utilising simultaneous equation and absorbance ratio method. The method is based on the measurement of absorbance of Febuxostat and Diclofenac at their respective wavelengths of 315 nm and 282nm and at the iso absorptive wavelength of 288 ...

We present procedures for determining theophylline in 50 mul of serum. The drug is extracted into a small volume of solvent that contains an internal standard, 8-chlorotheophylline. The extract is Analyzed by isocratic reversedphase chromatography, with measurement of eluted theophylline at 273 nm. Day-to-day reproducibility within 5% is attainable for the concentration range 5--20 mg/liter. Ot...

A novel, simple, rapid, precise, accurate, cost effective and reproducible spectrophotometric method has been developed for simultaneous estimation of ambroxol hcl and loratadine in combined tablet dosage form. The method employs measurement of absorbance at two wavelengths,308nm and 245nm, of ambroxol and loratadine respectively. Beer ́s law obeyed in the concentration range of 1050μg/ml and 10...

A new method is reported for the quantitative determination of glucuronic and galacturonic acid, which is based on spectrophotometric measurement of NADH. The NAD-linked oxidation of the uronic acids to the corresponding dicarboxylic acids is measured in the presence of uronic acid dehydrogenase. This enzyme was isolated from Pseudomonas syringae. The test is highly specific for glucuronic and ...

The purpose of this investigation was directed to propose sensitive, accurate and reproducible methods of analysis that can be applied to determine distigmine bromide (DTB), cyclopentolate hydrochloride (CPHC), diaveridine hydrochloride (DVHC) and tetrahydrozoline hydrochloride (THHC) drugs in pure form and pharmaceutical preparations via charge-transfer complex formation with 7,7,8,8-tetracyan...

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Montelukast sodium, Prasugrel, Ondensetron, Rosuvastatin calcium, Amlodepine besylate based on their reactivity towards Nbromosuccinimide (NBS). The method involves the addition of excess NBS of known concentration in the presence of 1M HCl, reactants are allowed to react and the unrea...

For the first time, we have implemented photoacoustic tomography (PAT) to image the water content of an edema in vivo. We produced and imaged a cold-induced cerebral edema transcranially, then obtained blood vessel and water accumulation images at 610 and 975 nm, respectively. We tracked the changes at 12, 24, and 36 h after the cold injury. The blood volume decreased after the cold injury, and...

Solubility measurements using chemiluminescent nitrogen detection (CLND) has advantages of reduced compound requirement and increased throughput compared to UV-spectrophotometric and HPLC-based measurements. CLND with direct flow injection was evaluated for the measurement of thermodynamic solubility to support drug discovery. The limit of quantitation (LOQ), accuracy, and day-to-day reproducib...

Spectrophotometric measurement applied on saturated granular activated carbon (GAC) is not yet explored. A colorimetric method in the visible range has been developed in order to determine the exhaustion level of GAC used in rum production. Aqueous ammonia solution has been used as an indicative agent to determine the extraction rate of taste compounds within the rum production process and the ...

A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utilit...