The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spe...

the aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (casa). the semen samples were collected from three ghezel rams. samples were diluted in seminal plasma (sp), phosphate-buffered saline (pbs) containing 1% bovine serum albumin (bsa) and bioexcell. the motility parameters that computed and rec...

Mammalian sperm mitochondrial sheath harbors a number of enzymes that are involved in release of energy via oxidative phosphorilation of ATP. The present study was designed to detect the effect of differential sperm mitochondrial activity on the fertility potential and the association of sperm mitochondrial activity index (SMAI) with the sperm motility parameters for cryopreserved semen samples...

A 2-step clustering procedure, using indexes derived from principal component analysis, was used to disclose sperm subpopulations within the canine ejaculate and its relationship to sperm cryoresistance. Semen from 4 dogs was frozen-thawed by a standard protocol: before freezing, computer-assisted sperm analysis of motility and morphometry were performed; after thawing, motility analysis was pe...

OBJECTIVE This study evaluated the integrity of the chromatin structure, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), DNA damage and lipid peroxidation of semen samples from infertile men classified as unexplained infertility. METHODS Between February 2010 and July 2011 semen parameters and functional tests were evaluated in 10 subjects with proven fertility, ...

background: atrazine (atr) is used as an agriculture herbicide worldwide. it has been shown that atr adversely affect the reproductive system in rodents. in this study we aimed to evaluate the impact of chronic exposure to atrazine (atr) on male testicular tissue, sperm parameters, serum level of total thiol molecules (ttm) and malodialdehyde (mda) content of testes. methods: to follow-up this ...

background: various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. according to literatures, it seems that hyaluronic acid (ha) has an important role on the permeability and motility of sperms and their interaction with gametes. objective: for evaluation of ha supplementation on sperm characteristics and fertilization capability, we investigated ...

We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study. Semen analysis and sperm DNA damage expressed as the DNA fragmentation index using acridine orange staining and chromatin condensation test by flow cytometry were assessed before and...

background: nano-particles are extensively employed in most industries. several studies have been started to explore the probable detrimental effects of nano-particles on human reproduction. however, there is insufficient and controversially evident of effects of silver nano-particles on sperm parameters and other reproductive indices. objective: investigation of the effects of silver nano-part...