Side effects of ciprofloxacin (CPFX), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. In the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm DNA damage using Aniline Blue and Acridine Orange technique, respectively. The fertility potential in male mice was also evaluated. NMRI male...

Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...

background: heat shock protein a2 (hspa2) is correlated with sperm maturity and function. therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. on the other hand, dna damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with dna fragmentation and chromatin abnormalities in human ejaculates is well documented,...

background: the availability of testis specific genes will be of help in choosing the most promising biomarkers for the detection of testicular sperm retrieval in patients with non-obstructive azoospermia (noa). testis specific chromodomain protein y 1 (cdy1) is a histone acetyltransferase which concentrates in the round spermatid nucleus, where histone hyperacetylation occurs and causes the re...

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 4...

abstract: background: the availability of testis specific genes will be of help in choosing the most promising biomarkers for the detection of testicular sperm retrieval in patients with non-obstructive azoospermia (noa). cdy1 histone acetyltransferase is a histone modifier which concentrates in the nucleus of round spermatids, where histone hyperacetylation occurs and causes the replacement of...

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...

Cysteine oxidation in protamines leads to their oligomerization and contributes to sperm chromatin compaction. Here we identify the Drosophila thioredoxin Deadhead (DHD) as the factor responsible for the reduction of intermolecular disulfide bonds in protamines and their eviction from sperm during fertilization. Protamine chaperone TAP/p32 dissociates DNA-protamine complexes in vitro only when ...

Meiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play dual function in determination of meiosis: 1) organizing a bipolar spindle formation and 2) indu...