OBJECTIVE The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. MATERIALS AND METHODS In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell® and tris-citric ...

Background: Vitamin E is believed to be the primary component of the antioxidant system in spermatozoa. There is a relationship between activity of these antioxidant and function of sperm. Vitamins E and C which are belong to non-enzymatic antioxidant are used as a supplemented drug to improve sperm quality and male fertility. The present study was carried out to investigate the positive effect...

objective: the aim of this study was to investigate the effects of curcumin on epididymal sperm parameters in adult male navel medical research institute (nmri) mice exposed to sodium arsenite. materials and methods: in this experimental study, we divided the animals into four groups: control, sodium arsenite (5 mg/kg), curcumin (100 mg/kg) and curcumin+sodium arsenite. exposures were performed...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

BACKGROUND Ketamine, an injectable anesthetic in human and animal medicine, is also a recreational drug used by young adults. The aim of this study is to evaluate the effects of ketamine on membrane integrity, DNA fragmentation and sperm parameters in humans. MATERIALS AND METHODS This prospective study was conducted on 40 males with normal semen samples over one month (August 2012). Subjects...

For long, spermatozoa were only considered as vectors carrying the paternal genetic material to the oocyte. However, over the recent years, the important contribution of the spermatozoa to embryogenesis has become better understood. It is now evident that sperm cells are highly differentiated, polarized and specialized, containing the paternal constituents required for fertilization and normal ...

Background: Antipsychotic medication use is a common cause of hyperprolactinemia that shown to have adverse effects on male fertility. Risperidone is a combined serotonin/dopamine receptor antagonist that can cause elevations in prolactine level. Its mechanism of cytotoxic effects on testicular germ cells is not fully understood. In the present study we sought to elucidate the impact of risperi...

OBJECTIVE To measure sperm DNA integrity values before and after varicocelectomy in patients with elevated preoperative levels of sperm DNA fragmentation. DESIGN Retrospective. SETTING Private urology clinic. PATIENT(S) Eleven patients with grade 1, 2, or 3 varicocele. INTERVENTION(S) Varicocelectomy. MAIN OUTCOME MEASURE(S) Sperm DNA fragmentation values were assessed before and afte...

We have recently presented evidence that the structural integrity of the mouse sperm nuclear matrix may be necessary for the proper unpackaging of sperm DNA for participation in embryogenesis. It is likely that the sperm nuclear matrix contributes to the organisation of the sperm DNA and its disturbance can seriously damage the paternal genome or its expression. In this work, we confirm our pre...

Background: Diabetes mellitus, a condition of chronic hyperglycemia, represents one of the greatest concerns to modern global health. Diabetes is a serious metabolic disorders with numerous complications. It is well known that, elevation of blood glucose levels leads to structural and functional changes in various target tissues and organs. The objective of the present study was to verify if th...