Piaractus orinoquensis is a native species from the Colombian Orinoquia and several studies have contributed to standardization of protocols for seminal cryopreservation this species. The main goal study was evaluate sperm motility, duration motility (DM), pH, membrane integrity (SMI) fertility semen cryopreserved seven years subjected different post-thaw storage times (PST). Semen five males (...

this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

background: it is established that sperm dna integrity is essential in fertilization and normal embryo and fetal development. routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm dna. therefore, the evaluation of sperm dna integrity, in addition to routine sperm parameters, could add further informat...

to compare the reproductive performance of the wild and cultured stocks of beluga, huso huso males, some spermatological parameters were measured including: sperm motility, sperm density, spermatocrit and also fertilization rate, hatching rate and larval growth and survival rates. the semen samples were sampled from 11 wild spawners and 12 cultured brooders. a half of each semen sample was allo...

backgroud: intrauterine insemination (iui) is generally attempted before proceeding to more expensive and invasive assisted reproductive techniques such as invitro fertilization (ivf) and intracytoplasmic sperm injection (icsi). this procedure is most commonly performed as a therapeutic method for couples with a wide variety of subfertility etiologies, such as low count or low motility of sperm...

Variations in the ejaculatory abstinence durations suggested by different guidance bodies have resulted in a growing concern among researchers over what the concise period of ejaculatory abstinence for an optimal semen sample ought to be. Several studies have been undertaken to examine the association between the length of sexual abstinence and semen characteristics. Not all studies, however, h...

BACKGROUND There is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status. Detecting such damage in sperm could provide new elements besides semen parameters in diagnosing male infertility. We aimed to assess sperm DNA fragmentation and oxidation and to study the association be...

Background: Fertilization and fecundation obviously are the phenomenon in which several factors are involved. Sperm membranous proteins are a series of these important elements. CD9, an inner acrosomal membrane protein, is one of the tetraspanin members that involved in sperm-egg fusion. CD52, is a GPI anchor protein which is expressed in the epididymal cells and during passage of sperms throug...

The aim of the present study was to assess if semen quality declines during in vitro fertilization (IVF) and whether or not this phenomenon is triggered by chronic male stress. In order to test this hypothesis, we first investigated a retrospective cohort of 155 male IVF patients (testing cohort). Subsequently, we started a prospective cohort study in men undergoing their first IVF and assessed...