Background: Antipsychotic medication use is a common cause of hyperprolactinemia that shown to have adverse effects on male fertility. Risperidone is a combined serotonin/dopamine receptor antagonist that can cause elevations in prolactine level. Its mechanism of cytotoxic effects on testicular germ cells is not fully understood. In the present study we sought to elucidate the impact of risperi...

High resolution electron micrographs of herring sperm protamine (clupeine) Y-I provide sufficient detail to constrain the folding of the known amino-acid sequence of the protein into a unique three-dimensional configuration. The structure consists of a loose helix of turns of various sizes held together at one edge, spread apart along the other. This form appears to represent the shape of sever...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Menezo and colleagues (1), in their commentary regarding the practice recommendations for sperm DNA fragmentation (SDF) testing based on clinical scenarios by Agarwal et al. (2), pointed to the importance of distinguishing between SDF and sperm nucleus decondensation (SND) as these are independent processes. The a...

BACKGROUND The aim of this study was to investigate the effects of curcumin on epididy- mal sperm parameters in adult male Navel Medical Research Institute (NMRI) mice ex- posed to sodium arsenite. MATERIALS AND METHODS In this experimental study, we divided the animals into four groups: control, sodium arsenite (5 mg/kg), curcumin (100 mg/kg) and curcumin+sodium arsenite. Exposures were perf...

A novel method for reconstituting sperm chromatin was used to investigate how protamine 1 condenses DNA. Complexes formed in vitro using linearized plasmid DNA were imaged and measured by atomic force microscopy (AFM). The structures formed were found to be highly dependent on the sample preparation method used for reconstitution. Interstrand, side-by-side fasiculation of DNA and toroidal-like ...

Protamines are expressed in the spermatid nucleus and allow denser packaging of DNA compared with histones. Disruption of the coding sequence of one allele of either protamine 1 (Prm1) or Prm2 results in failure to produce offspring, although sperm with disrupted Prm1 or Prm2 alleles are produced. Here, we produced Prm1-deficient female chimeric mice carrying Prm1-deficient oocytes. These mice ...