The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference in the qualities of semen diluted with two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender, between the 2, 8 or 12 and the 4°C control groups during storage for up to 48 hr, while ...

Mature ewes (12 per group) were given prostaglandin F20t (PG; 10 mg intramuscularly) on day 10 of an estrous cycle, to regress corpora lutea, or on day 16, after natural corpus luteum regression. Ewes treated on day 10 or 16 were in estrus on day 12 or 17, respectively. The PG-treated and untreated ewes were mated at estrus and necropsied 2 hr later. Sperm were counted in the oviducts, the uter...

For artificial insemination (AI), boar semen is prepared in rather simple extenders, kept at 16-20°C and usually used in liquid form for up to 3 days from extension. Routine freezing of boar semen is occasionally used for gene banking, replacement of lost genetics or long-distance transport [1]. The reason behind this meagre use of freezing is the low tolerance of the ejaculated boar sperm to c...

Acetylcholine, injected im into does immediately after natural mating, significantly increased the number of sperm recovered 1 h later from the vagina and cervices but not from the oviducts. Acetylcholine caused immediate increases in the frequency and amplitude of uterine contractions as measured by strain-gauge force transducers attached to the serosa and recorded by physiograph; contraction ...

Anion transport inhibitors, such as SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid) and heparin, inhibit reversibly the bicarbonate-sensitive adenylylcyclase of porcine sperm plasma membrane. In the light of this, SITS- and heparin-affinity chromatographies were applied in order to purify sperm adenylylcyclase. SITS-Affi-Gel 102 binds proteins extracted from the porcine cauda ep...

Semen analysis is the frontline and old reliable test in the assessment of male fertility, but it failed to provide absolute prediction of sperm function and its fertilizing ability. A wide variety of internal and external factors affect sperm function without discernible and significant effects on semen analysis. Oxygen toxicity and oxidative stress (OS) are inherent challenge of living cells ...

The human and murine genes for MRP9 (multidrug resistance-associated protein 9; ABCC12) yield many alternatively spliced RNAs. Using a panel of monoclonal antibodies, we detected full-length Mrp9 only in testicular germ cells and mouse sperm; we obtained no evidence for the existence of the truncated 100 kDa MRP9 protein reported previously. In contrast with other MRPs, neither murine Mrp9 nor ...

BACKGROUND Development of a non-hormonal long-acting reversible contraceptive for men could have a significant impact on reducing unintended pregnancies. Vasalgel™ is a high molecular weight polymer consisting of styrene-alt-maleic acid (SMA) dissolved in dimethyl sulfoxide being developed as a reversible male contraceptive device. It forms a hydrogel when implanted into the vasa deferentia, wh...

HOWARTH, B. 1971. Transport of spermatozoa in the reproductive tract of turkey hens. Poultry Sci. 50: 84-89. LAKE, P. 1975. Gamete production and the fertile period with particular reference to domesticated birds. Symp. Zool. Soc. London 35: 225-244. MERO, K. N., • F. X. OGASAWARA. 1970. Dimensions of uterovaginal sperm-storage tubules of the chicken and their possible significance in sperm rel...

The streaming endoplasm of characean cells has been shown to contain previously unreported endoplasmic filaments along which bending waves are observed under the light microscope using special techniques. The bending waves are similar to those propagated along sperm tails causing propulsion of sperm. In Nitella there is reason to believe that nearly all of the filaments are anchored in the cort...