Nanospray LC-MS analysis of dextromethorphan (DEM) and its metabolites was performed with the Agilent 1200 Series HPLC-Chip/TOF system. Automated nanospray HPLC-Chips with the high sensitivity TOF allowed detection of all DEM metabolites, following incubation at concentration levels 100-fold lower than normally used. In addition, system response was linear over a wide concentration range for me...

Abstract Background Glyphosate is the declared active component of most extensively used herbicides in world, and therefore widely present environment. urinary levels represent a relevant biomarker for each individual’s exposure to glyphosate-based herbicides. However, GLY urine level measurement controversial because different detection methods have led contradictory results, especially cases ...

During the last few years γ-hydroxybutyric acid (GHB) and γ-butyrolactone (GBL) have attracted much interest as recreational drugs and knock-out drops in drug-facilitated sexual assaults. This experiment aims at getting an insight into the pharmacokinetics of GHB after intake of GBL. Therefore Two volunteers took a single dose of 1.5 ml GBL, which had been spiked to a soft drink. Assuming that ...

Cephapirin, a cephalosporin antibiotic, is used by the majority of dairy farms in the US. Fecal and urinary excretion of cephapirin could introduce this compound into the environment when manure is land applied as fertilizer, and may cause development of bacterial resistance to antibiotics critical for human health. The environmental loading of cephapirin by the livestock industry remains un-as...

The consumption of poppy seeds in various foods may lead to a positive opiate result in urine subjected to testing for drugs of abuse. As a natural constituent of poppy seeds, thebaine was investigated as a possible marker for poppy seed consumption. Poppy seeds were examined for opiate content by gas chromatography-ion trap mass spectrometry (GC-MS) after extraction with methanol. Urine sample...

      Metformin hydrochloride (MFH) is an oral anti-diabetic drug of biguanide class. Two selective spectrophotometric methods were presented for the determination of MFH in pharmaceuticals. The methods were based on the measurement of yellow-coloured charge-transfer complexes formed between MFH and two poly-nitrophenols, namely, 2,4-dinitrophenol (DNP method, at 405 nm) and picric acid (PA met...

The study reports quantification of metronidazole (MTZ) in clinical human urine employing our innovative stochastic dynamic equation D”SD =2.6388.10-17.(–). There are direct analysis without presence internal standard, using ultra-high accuracy nano-electrospray ionization (ESI) mass spectrometry (MS), quantum chemistry and chemometrics, respectively. linear calibration =f(conc.) equati...

Supercritical fluid extraction (SFE) is emerging as a valuable analytical technique for use as an alternative to conventional solid-phase (SPE) and liquid-liquid extraction techniques. It is a relatively new technique based on the use of supercritical fluids for the isolation of analytes from various matrices and is attracting great interest because of the increasing need for a simple, rapid, e...