Highly active cytochrome b6f complexes from spinach and the cyanobacterium Mastigocladus laminosus have been analyzed by liquid chromatography with electrospray ionization mass spectrometry (LCMS ). Both sizeexclusion and reverse-phase separations were used to separate protein subunits allowing measurement of their molecular masses to an accuracy exceeding 0.01% ( 3 Da at 30,000 Da). The produc...

The agriculture sector is known to be the one of the major contributors to global greenhouse gas (GHG) emissions. At the same time, global climate changes have affected the agriculture sector. In order to strengthen the sustainable development of agriculture, it is important to promote environmentally friendly farming and simultaneously increase the economic value. To improve the productivity o...

We designed an in vitro signal amplification circuit that takes a short RNA input that catalytically activates the Spinach RNA aptamer to produce a fluorescent output. The circuit consists of three RNA strands: an internally blocked Spinach aptamer, a fuel strand, and an input strand (catalyst), as well as the Spinach aptamer ligand 3,5-difluoro-4-hydroxylbenzylidene imidazolinone (DFHBI). The ...

The present study is an attempt to investigate the radioprotective efficacy of spinach against radiation induced oxidative stress, since its leaves are rich in antioxidants like carotenoids (beta-carotene, lutein and zeaxanthin) and high content of proteins, minerals, vitamin C. For the experimental study, healthy Swiss mice were selected from an inbred colony and divided into four groups. Grou...

Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability. Folate absorption was determined in healthy ileostomy v...

Campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. We have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. Carbon utilization profiles revealed that C. jejuni can utilize many organic ac...

The effects of chilling temperatures, in light or dark, on the isolated thylakoids and leaf discs of cucumber (Cucumis sativa L. "Marketer") and spinach (Spinacia oleracea L. "Bloomsdale") were studied. The pretreatment of isolated thylakoids and leaf discs at 4 C in the dark did not affect the phenazine methosulfate-dependent phosphorylation, proton uptake, osmotic response to sucrose, Ca(2+)-...

In previous reports large-scale EDTA-based soil washing using ReSoil® technology was demonstrated. the current study, we established a vegetable garden with nine raised beds (4 × 1 0.5 m), three original (contaminated) soil, remediated and vitalized by addition of vermicompost, earthworms, rhizosphere inoculum. The managed in 6 rotations between July 2018 November 2019. Buckwheat sown first as ...

Extractability of protein in water spinach (Ipomoea aquatica Forsk., I. reptans Poir), a possible source of food protein concentrate was examined. Fresh and fresh frozen leaves were extracted by the conventional bufferhomogenate method at room temperature and by a method involving pre-treatment of the leaves with polar and nonpolar solvents. Pre-treatment of leaves with dimethylsulfoxide (DMSO)...

Background: Replacement of the gluten is one of the challenging issues in the food industry, since producing nutritious and functionally acceptable gluten-free foods is difficult. The cake is a cereal-based product that attracts the consumers due to its various flavors, long shelf-life, and relatively low cost. Considering the competition in markets and the increased demand for functional and n...