© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Adv. Funct. Mater. 2011, XX, 1–10 DOI: 10.1002/adfm.201101023 A spatially organized three-dimensional (3D) co-culture of multiple cell types is required to recapitulate cellular interactions and microenvironments in complex tissues. Although there are limited reports for 3D patterning of cells and materials, approaches to examine functional inte...