BACKGROUND Debridement is essential for successful wound management. Enzymatic debridement is commonly utilized in wound care but has been reported to be unsafe in wounds with significant bacterial bioburden, unless used in conjunction with topical antimicrobials. We examine this hypothesis with 2 commercially available, commonly used preparations of enzymatic debriding agents. MATERIALS AND ...

This study investigated the effect of copper as an antibacterial agent on the infectivity of Salmonella enterica serovar Typhimurium. Mice were infected orally with a standardized dose of unstressed Salmonella Typhimurium and copper-stressed cells of Salmonella Typhimurium. Bacterial counts in ileum, blood, liver and spleen were observed up to 168 h under normal aerobic conditions. Serum sensit...

The gut microbiota can contribute to host physiology leading an increase of resistance abiotic stress conditions. For instance, temperature has profound effects on ectotherms, and the role thermal tolerance ectotherms is a matter recent research. However, most these studies have been focused single static temperatures instead evaluating in wide range stressful temperatures. Additionally, there ...

AIMS To assess the ability of human immunoglobulin Fc fragment binding activity to differentiate human biotype large colony group G streptococci from the group G "Streptococcus milleri group". METHODS Fifty two isolates of large colony group G streptococci and 30 group G "S milleri group" strains were tested for their ability to bind fluorescein conjugated human IgG Fc fragments after acetone...

the aim of this study was to assess the effects of nucleotide on intestinal microbiota of striped catfish, pangasianodon hypophthalmus . different levels of the nt (0, 0.25. 0.5, 0.75 and 1 % weight per weight, ww -1 ) were added to basal diet and then randomly allocated to triplicate groups of fish with average initial weight (1.52±0.11 g) for 10 weeks. at the end of the experiment, at least 1...

Bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. However, most such proteins accumulate in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) in E. coli. In this repor...

The radiometric method has been used to detect the presence of bacteria in serial dilutions of nine bacterial species with and without agitation. It was also found that the detection time is proportional to the dilution showing high sensitivity down to one colony forming unit and the agitation resulted in different manners depending upon bacterial species (JPMA 37 : 34 , 1987).

We provide a model for induction of T cell-independent, polysaccharide-specific Ig secretion in response to bacterial challenge. Two predominant pathways are defined that require the concerted action of multivalent membrane Ig cross-linking by the polysaccharide Ag with 1) various B cell-activating moieties contained within the bacterial pathogen and/or 2) cytokines, such as IFN-gamma and granu...