We report here the ultrastructural organization of collagen fibrils (CF) and proteoglycans (PGs) of the corneal stroma of both the stingray and the shark. Three corneas from three stingrays and three corneas from three sharks were processed for electron microscopy. Tissues were embedded in TAAB 031 resin. The corneal stroma of both the stingray and shark consisted of parallel running lamellae o...

It is widely believed that benign prostatic hyperplasia (BPH) is associated with aging. Thus, the question arises whether or not a correlation exists between the well known prostatic androgen and estrogen accumulation and aging. To address this question, we measured 5 alpha-dihydrotestosterone (DHT), testosterone, estradiol, and estrone in epithelium and stroma of six normal (NPR) and 19 BPH an...

Studies on hematopoiesis have focused on the function and composition of human bone marrow stroma. Stroma function gives hematopoietic stem cells the microenvironment appropriate for self-renewal and/or prompt differentiation into hematopoietic progenitor cells, then into terminal specialized cells. Human bone marrow stroma has been dissected into hematopoietic and nonhematopoietic components. ...

Potato branching enzyme, a key enzyme in the biosynthesis of starch, was localized in amyloplasts in starch-storage cells of potato (Solanum tuberosum L.) with the use of immunogold electron microscopy. Branching enzyme was found in the amyloplast stroma, concentrated at the interface of the stroma and the surface of the starch granule. ADP-glucose pyrophosphorylase, a key regulatory enzyme in ...

To date, methods for quantifying cellular proliferation within the human ovary have relied primarily on static measurements at time of tissue recovery, such as labeling ki67 and phosphohistone-H3. Here, we describe a method based consecutive injection two thymidine analogues, ethynyl deoxyuridine (EdU) 5-chloro-2′-deoxyuridine (CldU), in ovarian xenograft model, allowing resolution division dis...

To develop a microfluidic cervix-on-a-chip (COC) to study ascending bacterial infection in pregnancy. COC with 6 interconnected culture chambers connected by microchannels containing cells from vagina (VEC), cervical epithelium and stroma, transition zone, decidua was developed. Cells were characterized morphology expression of cell-specific markers. An Ureaplasma parvum (UP) tested inoculating...

Abstract Background Invasive micropapillary carcinoma (IMPC) is a rare malignant breast tumor and variant form of invasive ductal that an aggressive neoplasm the human canine mammary gland. The importance microenvironment in cancer development has gradually been recognized, but little known about cell types outlining cystic space IMPC. This study aimed to characterize neoplastic cells Results I...

Abstract TFE3 -translocation renal cell carcinoma ( -tRCC) is a rare and heterogeneous subtype of kidney cancer with no standard treatment for advanced disease. We describe comprehensive molecular characteristics 63 untreated primary -tRCCs based on whole-exome RNA sequencing. -tRCC highly heterogeneous, both clinicopathologically genotypically. ASPSCR1-TFE3 fusion several somatic copy number a...

The present study aims to clarify corneal development via analyzing of the histomorphologic and some histochemical parameters in local rabbits. Samples were collected from 25 Rabbits divided into five different age groups at 1, 10, 15, 30, 40 postnatal day (PND), then samples sectioned, processed, stained with Hematoxylin eosin stain Masson’s trichrome stain. Some sections further periodic acid...