This study reports on the potential of using ion-exchange suppressor technology in liquid chromatography—electrospray ionization mass spectrometry workflows. The aim was to use high salt concentrations improve separation performance, while overcoming resulting significant ion suppression during electrospray ionization. As a case study, we apply hydrophilic interaction chromatography and detecti...

Previous studies on rabbit thrombomodulin (TM) revealed that certain anticoagulant activities expressed by TM depend on the presence of an acidic domain tentatively identified as a sulfated galactosaminoglycan (Bourin, M.-C., Ohlin, A.-K., Lane, D., Stenflo, J., and Lindahl, U. (1988) J. Biol. Chem. 263, 8044-8052). The glycan was released by alkaline beta-elimination, isolated by ion-exchange ...

This article reports the ginger protease extraction and purification methods from fresh ginger rhizome. As to ginger protease extraction, we adapt the steps of organic solvent dissolving, ammonium sulfate depositing and freeze-drying, and this method can attain crude enzyme powder 0.6% weight of fresh ginger rhizome. The purification part in this study includes two steps: cellulose ion exchange...

Twelve Hereford-Angus weanling heifers were used in a split:plot design incorporating a randomized complete block to determine physiological effects of subtoxic concentrations of inorganic sulfate in drinking water. Treatments were tap-water (110 mg/liter sulfate), 1,250 rag/liter sulfate and 2,500 rag/liter surface. The sulfate was added to the tap water as sodium sulfate. The sulfate-waters d...

Based on the principles of mass conservation, chemical equilibrium, and electron charge neutrality, a thermodynamic equilibrium system was established for nickel-cobalt-manganese sulfate leaching solution in recovery process spent lithium-ion batteries. By changing ion concentration system, calculating pH value, identifying complexes Cu2+, Fe3+, PO43−, Al3+, F− results were obtained used to dra...

Chondroitin sulfate (CS) has been accepted as an ingredient in health foods for the treatment of symptoms related to arthritis and cartilage repair. However, CS is poorly absorbed through the gastrointestinal tract because of its high negative electric charges and molecular weight (MW). In this study, poly-ion complex (PIC) formation was found in aqueous solutions through electrostatic interact...

A simple analytical system suitable for nephelometric measurements is introduced in this article. The electronic and optical components are simplified so that the apparatus is easily assembled from materials that are generally available. This instrument is designed for the efficient measurement of anions, and microgram quantities of ions may be determined by a convenient method of titration...

The biosynthesis of heparan sulfate, present on the cell surface and in the basal lamina surrounding cells, is a multistep process in which each step is mediated by a specific enzyme. The initial modification of the precursor polysaccharide, N-deacetylation followed by N-sulfation of selected N-acetyl-D-glucosamine residues, is catalyzed by the enzyme glucosaminyl N-deacetylase/N-sulfotransfera...

The anions chloride, sulfate and nitrate in nearly 500 pairs of ice core samples from the same depth were determined in a 121 m long ice core from Svalbard. The analyses were performed separately using an ion chromatograph with Dionex AS9 and AS15 columns with Na(2)CO(3) and NaOH eluents. Results showed a small (5-6 microg l(-1)) but statistically significant difference in mean concentrations f...