In this work we report that superoxide dismutase is entrapped in a microbody-like organelle, the glycosome, present in Leishmania spp. Studies on the sensitivity of the enzyme to various inhibitors indicated that glycosomal superoxide dismutase is predominantly of the Cu/Zu type. Localization of superoxide dismutase in glycosomes points to the major importance of this organelle in the survival ...

In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing nativ...

The activity of eleven enzymes involved in development, ageing and in metabolism of xenobiotics in insects, was investigated under the influence of 2,4-D (2,4-Dichlorophenoxyacetic acid), in the mustard aphid, Lipaphis erysimi (Kaltenbach) (Rhynchota Aphididae), after treating the 2 instar nymphs for 13, 25 and 37 h. Studies of two hydrolases and one group transfer enzyme revealed significant e...

Drought is one of the most important environmental factors limiting the cultivation of ornamental plants in the green space. The effects of spermidine and salicylic acid on hollyhock (Alcea rosea L.)under drought stress were investigated. At first, the plants were sprayed with 3 doses of spermidine and 3 doses of salicylic acid (control, 100, 200 and 400 μM) for three consecut...

Babesia hylomysci and B. divergens were studied for superoxide dismutase (SOD) activity by enzyme assay and isoelectric focusing (IEF). In the two Babesia species, parasite-associated SOD is cyanide-insensitive and inhibited by H2O2, indicating that iron is the cofactor metal. Measurements of SOD activity from purified parasites show that the SOD activity detected in Babesia is, for the main pa...

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese Superoxide dismutase, was transfected with a human manganese Superoxide dismutase complementary DNA. Fifty clones were screened for manganese Superoxide dismutase activity by the Superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction a...

the present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (i/r) injury in rats.forty male rats were randomly divided into sham, control (i/r injury) and three crocin-pretreated groups. to induce i/r lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. pretreated-rats rec...

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese superoxide dismutase, was transfected with a human manganese superoxide dismutase complementary DNA. Fifty clones were screened for manganese superoxide dismutase activity by the superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction a...

The Cu,Zn superoxide dismutase (SOD1) is an ubiquitary cytosolic dimeric carbohydrate free molecule, belonging to a family of isoenzymes involved in the scavenger of superoxide anions. This effect certainly represents the main and well known function ascribed to this enzyme. Here we highlight new aspects of SOD1 physiology that point out some inedited effects of this enzyme in addition to the c...

Abstract The objective of this work was to validate the enzymatic activity in Annona x atemoyaseeds submitted treatments with plant growth regulators belonging group gibberellins and cytokinins for overcoming dormancy during germination process. Initially, water acquisition curve determined, where two points phase II (72 144 hours) were determined evaluate superoxide dismutase (SOD), peroxidase...