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Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralised tissues is more complicated than with soft tissues. This can in most cases be overcome by demineralisin...
The nature, biological characteristics, and contribution to organ physiology of skeletal stem cells are not completely determined. Chan et al. and Worthley et al. demonstrate that a stem cell for skeletal tissues, and a system of more restricted, downstream progenitors, can be identified in mice and demonstrate its role in skeletal tissue maintenance and regeneration.
Integration of muscle, connective tissue and skeletal patterning during development is essential for proper functioning of the musculoskeletal system. How this integration is achieved is poorly understood. There is ample evidence suggesting that skeletal pattern is programmed autonomously, whereas muscle pattern is, for the most part, programmed non-cell-autonomously. Connective tissues depend ...
This review reports on proceedings of a bone histomorphometry session conducted at the Fortieth International IBMS Sun Valley Skeletal Tissue Biology Workshop held on August 1, 2010. The session was prompted by recent technical problems encountered in conducting histomorphometry on bone biopsies from humans and animals treated with anti-remodeling agents such as bisphosphonates and RANKL antibo...
The topological architecture and functions of different cells of the olfactory epithelium in Wallago attu (Bleeker) have been systematically studied using a scanning electron microscope. The elongated olfactory rosette of the fish consists of 62 to 64 primary lamellae in each left and right rosette. Each lamella is provided with apical sensory epithelium and basal non-sensory epithelium. Topolo...
1 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49 PB813, B-3000 Leuven, Belgium 2 Dept. Development and Regeneration Skeletal Biology and Engineering Research Center, KU Leuven, O&N 1, Herestraat 49 PB813, B-3000 Leuven, Belgium 3 Dept. Chemistry Molecular Design and Synthesis, KU Leuven, Celestijnenlaan 200f – PB2404, B-3001 Leuven, Belgium 4 Dept. Clinical...
1 Department of Metallurgy and Materials Engineering, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium; [email protected], [email protected], [email protected], [email protected], [email protected] 2 Department of Mechanical Engineering, Division of Production engineering, Machine design and Automation, Katholieke Universiteit...
The INHAND (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) Project (www.toxpath.org/inhand.asp) is an initiative of the Societies of Toxicological Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP) and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laborato...
We describe a new type of composite skeletal tissues in which calcite and stabilized amorphous calcium carbonate (ACC) coexist in well-defined domains. The organisms that form such structures are widely separated in the animal kingdom phylogenetic tree: calcareous sponges and ascidians. This paper compares the microstructures of their composite skeletal elements: The triradiate spicules from th...
appendix, over-all this volume deserves nothing but praise. It is an absolute "must" for anyone who enters this complicated field. Moreover, the organization of the book is such that the casual reader seeking an isolated bit of information will be as well served as he who sits and studies it intensively. The book is highly recommended to anyone whose interests include an understanding of the bi...
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