نتایج جستجو برای: sybr
تعداد نتایج: 1543 فیلتر نتایج به سال:
High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane fi...
Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethi...
PE Biosystems has developed guidelines enabling streamlined design and implementation of real-time quantitative PCR assays. The use of these guidelines makes it easy to apply either the fluorogenic 5 ́ nuclease assay or SYBR Green I double-stranded DNA binding dye chemistry to any real-time quantitative PCR system. Specific assay design and optimization guidelines minimize the time and cost of a...
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