نتایج جستجو برای: sybr green real time

تعداد نتایج: 2267419  

Journal: :Polish Journal of Veterinary Sciences 2023

In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on highly conserved region within Capsid gene PPV7. The established was 1,000 times more than conventional had limit 35.6 copies. This specific no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, PPV6. Experiments testi...

Journal: :Molecular reproduction and development 2005
Yu-Jeong Jeong Hye-Won Choi Hyeon-Sang Shin Xiang-Shun Cui Nam-Hyung Kim George L Gerton Jin Hyun Jun

This study was carried out to optimize conditions for using real time RT-PCR as an efficient and precise quantitative method for estimating the transcript levels of genes expressed in samples containing miniscule amounts of RNA, such as single mammalian oocytes and embryos. First, using mouse eggs and blastocysts, we tested three kinds of RNA isolation or collection methods: TRIZOL reagent, oli...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید