Flavodiiron (Flv) proteins are involved in detoxification of O(2) and NO in anaerobic bacteria and archaea. Cyanobacterial Flv proteins, on the contrary, function in oxygenic environment and possess an extra NAD(P)H:flavin oxidoreductase module. Synechocystis sp. PCC 6803 has four genes (sll1521, sll0219, sll0550, and sll0217) encoding Flv proteins (Flv1, Flv2, Flv3, and Flv4). Previous in vitr...

Phycobilisomes, the light harvesting complexes of cyanobacteria are highly mobile, fluorescent complexes known to diffuse freely on the thylakoid membranes, interacting with the reaction centre complexes to mediate efficient photosynthesis. The primary aim of this project is to establish what processes require this rapid movement of the complexes using a number of genetic, biochemical and micro...

Femtosecond photodynamics of the Pfr form of the red/far-red phytochrome N-terminal PAS-GAF-PHY photosensory core module of the cyanobacterial phytochrome Cph1 (termed Cph1Δ) from Synechocystis were resolved with visible broadband transient absorption spectroscopy. Multiphasic generation dynamics via global target analysis revealed parallel evolution of two pathways with distinct excited- and g...

Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (D.J.L-S., D.S.B, C.J.H.); Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA United Kingdom (N.R., M.Z., A.G.S.); Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB2 3RA United Kingdom (N.R., J.S.D.); and Department of Engineering, University o...

Englund, E. 2016. Metabolic Engineering of Synechocystis sp. PCC 6803 for Terpenoid Production. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1455. 63 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9761-3. In the Paris Agreement from 2015, nations agreed to limit the effects of global warming to well below 2°C. To be able to re...

BACKGROUND The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of product is a key element in the manipulation of this organism. ...

The photosynthetic cyanobacterium Synechocystis sp. strain PCC 6803 uses a complex genetic program to control its physiological response to alternating light conditions. To study this regulatory program time-series experiments were conducted by exposing Synechocystis sp. to serial perturbations in light intensity. In each experiment whole-genome DNA microarrays were used to monitor gene transcr...