Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum cou...

Objective(s): Umbilical cord blood is a good source of the mesenchymal stem cells that can be banked, expanded and used in regenerative medicine.  The objective of this study was to test whether amniotic membrane extract, as a rich source of growth factors such as basic-fibroblast growth factor, can promote the proliferation potential of the umbilical cord mesenchymal stem cells. Materials and ...

Purpose: To investigate the function and interaction of heat-shock cognate protein 70 (Hsc70) potassium voltage-gated channel subfamily A member 5 (KCNA5) in polycystic ovary syndrome (PCOS).Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) western blot were used to measure Hsc70 KCNA5 expression PCOS. The effect on granulosa cell apoptosis was determined by flow c...

Background  Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type I gene ...

objective(s):in previous investigations, we have shown para-nonylphenol (p-np) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (mscs) in vitro. in this study, we first treat the rats with p-np, then carried out the biochemical and morphological studies on mscs. materials and methods: proliferation property of cells was evaluated with t...

chemical investigation of garcinia eugenifolia and calophyllum enervosum yielded six compounds. one of these was found to be a novel compound identified as enervosanone. five known compounds cambogin, epicatechin, osajaxanthone, rubraxanthone and isocowanol, were also isolated. these compounds were tested for their bioactivity as antimicrobial, antioxidant and cytotoxicagents. antimicrobial ass...

Background: Peripheral nerve injury (PNI) is a common condition that compromises motor and sensory functions. Peripheral nerves are known to have regenerative capability and the pineal hormone, melatonin, is known to aid nerve regeneration. However, the role of Schwann cells and the pathways involved remain unclear. Thus, the aim of this study is to identify the effects of melatonin on Schwann ...

background: several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (mscs) in allogeneic or mitogenic interactions. cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. objective: to investigate if adipose derived mscs could inhibit jurkat lym-phoblastic leukemia t cell proliferation during coculture. me...

background: the health risks of crack cocaine smoking on the oral mucosa has not been widely researched and documented. objective: the purpose of this study was to analyze the proliferative activity of oral epithelial cells exposed to crack cocaine smoke using silver nucleolar organizer region (agnor) staining. methods: oral smears were collected from clinically normal-appearing buccal mucosa b...

a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...