The liver of the guinea pig was shown (1, 2) to contain two enzymes which catalyze the dehydrogenation of testosterone. One is located in the particulate fraction and specifically requires diphosphopyridine nucleotide as hydrogen acceptor; the other, present in the fraction of guinea pig homogenate not sedimented by 24,500 X g for 60 minutes, specifically requires triphosphopyridine nucleotide ...

BACKGROUND Bortezomib is an FDA-approved proteasome inhibitor, and oncolytic herpes simplex virus-1 (oHSV) is a promising therapeutic approach for cancer. We tested the impact of combining bortezomib with oHSV for antitumor efficacy. EXPERIMENTAL DESIGN The synergistic interaction between oHSV and bortezomib was calculated using Chou-Talalay analysis. Viral replication was evaluated using pla...

PURPOSE To investigate the effect and related molecular mechanisms of lapatinib/celastrol combination or single-agent treatment in HER2/neu-overexpressing MDA-MB-453 breast cancer cells. METHODS The effects of treatment with lapatinib, celastrol or their combination on cell growth were determined using MTT assay. Drug synergy was determined using the combination index (CI) methods derived fro...

This article represents the main positions of the theory of pleiotropic action of biologically active compounds (BACs) and medicines, which has been designed by the author based on her own experimental researches. The term “pleiotropy” means the ability of the BACs and medicines to implement more than one mechanism of action resulting in the specific biological (pharmacological) effect. The int...

PURPOSE There is an opportunity to augment the therapeutic potential of drug combinations through use of drug delivery technology. This report summarizes data obtained using a novel liposomal formulation with coencapsulated doxorubicin and vincristine. The rationale for selecting these drugs is due in part to the fact that liposomal formulations of doxorubicin and vincristine are being separate...

Introduction Intake of broccoli sprouts, a rich source of the glucosinolate glucoraphanin, has been associated with decreased incidence, multiplicity, and tumor growth in animal cancer models.1-3 In 1992, Paul Talalay, MD, and colleagues at Johns Hopkins University identified the isothiocyanate, sulforaphane, a biologically active metabolite of glucoraphanin, as the compound in broccoli respons...

This now famous quote neatly captures the confusion and uncertainty surrounding the transgressive character of the action heroine.' Smarter, tougher and better equipped than both the traditional heroines of the action genre and many of their contemporary male counterparts, action heroines are a new breed of arse-kicking female protagonists in action genre films Aggressive, heroic and transforma...

A promising new strategy for cancer therapy is to target the autophagic pathway. In the current study, we demonstrate that the antimalarial drug Quinacrine (QC) reduces cell viability and promotes chemotherapy-induced cell death in an autophagy-dependent manner more extensively in chemoresistant cells compared to their isogenic chemosensitive control cells as quantified by the Chou-Talalay meth...

Purpose:Oncolytic viruses (OV) based on herpes simplex virus type 1 (HSV1) are being used in clinical trials for a variety of cancers. The OV, rQNestin34.5, uses a nestin promoter/enhancer to selectively drive robust viral replication in malignant glioma cells. We have discovered that this promoter becomes extensively methylated in infected glioma cells, reducing OV efficacy. ExperimentalDesign...

Hematological malignancies are important diseases that need more powerful therapeutics. Even with current targeting therapies, such as rituximab and other chemotherapeutic agents, there is a need to develop new treatment strategies. Combination therapy seems the best option to target the tumor cells by different mechanisms. Virotherapy is a very promising treatment modality, as it is selective,...