Abbreviations used: Acm, acetamidomethyl; Boc, tert-butoxycarbonyl; Bzl, benzyl; Bzl(4-Me), 4-methylbenzyl; DPDS, di(2-pyridyl)disulfide; DMSO, dimethylsulfoxide; Fmoc, 9-fluorenylmethoxycarbonyl; Mob, 4-methoxybenzyl; Npys, 3-nitro-2-pyridylsulfanyl; Pys, 2-pyridylsulfanyl; StBu, tert-butylsulfanyl; sec, selenocysteine; tBu, tert-butyl; TFA, trifluoroacetic acid; TfOH, trifluoromethanesulfonic...

The valproic acid (VPA)-induced animal model is one of the most widely utilized environmental risk factor models of autism. Autism spectrum disorder (ASD) remains an insurmountable challenge among neurodevelopmental disorders due to its heterogeneity, unresolved pathological pathways and lack of treatment. We previously reported that VPA-exposed rats and cultured rat primary neurons have increa...

BACKGROUND Telomerase is an enzyme that catalyzes the addition of nucleotides on the ends of chromosomes. Rare loss of function mutations in the gene that encodes the protein component of telomerase (TERT) have been described in patients with idiopathic pulmonary fibrosis (IPF). Here we examine the telomere lengths and pulmonary fibrosis phenotype seen in multiple kindreds with heterozygous TER...

(2S,4R)- and (2S,4S)-perfluoro-tert-butyl 4-hydroxyproline were synthesized (as Fmoc-, Boc-, and free amino acids) in 2-5 steps. The key step of each synthesis was a Mitsunobu reaction with perfluoro-tert-butanol, which incorporated a perfluoro-tert-butyl group, with nine chemically equivalent fluorines. Both amino acids were incorporated in model α-helical and polyproline helix peptides. Each ...

Highly regioselective dialkylation of naphthalene using various alkylating agents can be achieved over zeolite catalysts. For example, the tert-butylation of naphthalene (1) using tert-butanol in cyclohexane over a dealuminated H-Mordenite (HM) zeolite has been optimised to give a 60% yield of 2.6-di-tert-butylnaphthalene (3) with a 2.6/2.7 ratio of over 50. This has been achieved by varying th...

The first mixed phosphatetrahedranes were reported only recently and their reactivity is virtually unexplored. Herein, we present a study on di-tert-butyldiphosphatetrahedrane (1), which the dimer of tert-butylphosphaalkyne. (tBuCP)2 tetrahedron activated selectively by N-heterocyclic carbene (NHC) nickel(I) nickel(0) complexes, resulting in novel complexes featuring diverse (tBuCP)n-frameworks...

OBJECTIVE To gain insights into mechanisms by which intimal hyperplasia interferes with the repair process by investigating expression and function of the catalytic telomerase reverse transcriptase (TERT) subunit after vascular injury. METHODS AND RESULTS Functional telomerase is essential to the replicative longevity of vascular cells. We found that TERT was de novo activated in the intima o...

We investigated whether glioma stem-like cells (GSCs) malignantly transformed bone marrow mesenchymal stem cells (tBMSCs) in the tumor microenvironment. Transplantation of enhanced green fluorescence protein (EGFP)-labeled BMSCs into irradiated athymic nude mice was followed by intracranial injection of red fluorescent protein-expressing glioma stem-like cells (SU3-RFP-GSCs). Singly cloned EGFP...

BACKGROUND The BRAF V600E and telomerase reverse transcriptase (TERT) promoter mutations have been reported in papillary thyroid carcinoma (PTC). The aim of this retrospective cross-sectional study was to add further information regarding the prevalence of the BRAF V600E and TERT promoter mutations in Chinese PTC and their clinicopathological associations. METHODS We detected the BRAF V600E m...