This study describes a multistage methodology to detect minute amounts of tetrodotoxin in fishes, plan that may be broadened include other marine organisms. was applied porcupinefish (Diodon hystrix) collected Punta Chiquirín, El Salvador. A three-stage approach along with post-acquisition processing employed, wit: (a) Sample screening by selected reaction monitoring (HPLC-MS/MS-SRM) analyses q...