Over a 26 month period 17% of couples having treatment in our clinical programmes selected a commercially available protein (normal serum albumin, NSA) prepared from pooled human sera instead of using their own serum as a supplement for their embryo culture media. In a retrospective analysis of >2000 gonadotrophin-stimulated cycles and 1000 cycles where frozen/thawed embryos were transferred, f...

EFFICACY OF INTRAUTERINE INSTILLATION AUTOLOGOUS PLATELET-RICH PLASMA ON ENDOMETRIAL GROWTH DURING THE FROZEN-THAWED EMBRYO TRANSFER CYCLES

objective: we evaluated structural and functional changes of fresh and frozen-thawed adult mouse spermatogonial stem cells following auto-transplantation into gamma-irradiated testes. materials and methods: in this experimental research, the right testes from adult mice (n=25) were collected, then sertoli and spermatogonial cells were isolated using two-step enzymatic digestion, lectin immobili...

Study question: Does the type of medium used to culture fresh and frozen-thawed embryos influence neonatal birthweight after single embryo transfer (SET) in IVF? Summary answer: A comparison of two commercially available culture media showed no significant influence on mean birthweight and mean birthweight adjusted for gestational age, gender and parity (z-scores) of singletons born after a fre...

To the Editor: The recent introduction of a general coagulation function test, namely the thrombin-generation assay (TGA), has enabled efficient assessment of the global functioning of the hemo-static system. By using a fluorogenic substrate, the TGA produces throm-bin-generation curves in a fully automated manner that may be useful and sensitive enough to screen for either hypercoagulable stat...

Freezing action always results in great damage to the pore structure and fractal characteristics of freezing–thawing soft soil. Nuclear magnetic resonance (NMR) can help collect data describe microstructure frozen–thawed This paper conducted a series nuclear tests interpret effect freezing duration, pressure temperature on soil’s micro-pore characteristics. The size distributions (PSDs) tested ...

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...

degradation of dry matter (dm) and nitrogen (n) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. the forages were labeled with 15n during growth in a glasshouse, harvested at similar growth phase, and fresh (f) or frozen-thawed (ft) samples were incubated in the rumen of 3 sheep. there was no difference (p>0.05) between forage type (s) for the immediately s...

Effects of Extrinsic Selenium Nanoparticles on the Qualitative Parameters Frozen-Thawed Sperm Broiler Breeder Roosters under Oxidative Stress Conditions

semen from six adult male black bengal goats (capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. each collected samples was divided into two parts: one part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚c) and equilibrated for freezing.in both cases, semen was dilu...