A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acros...

1. The rigor which takes place when completely frozen frog sartorius muscle is thawed ("thaw rigor"), is accompanied by a decrease in length of 70 per cent and a loss in weight of 35 per cent, whether the muscle is frozen in the resting or the exhausted condition, or during isometric tetanus. Muscle tetanized to maximal shortening shows a loss in weight of 25 per cent on thawing. 2. A load of 8...

OBJECTIVE To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. DESIGN Prospective-controlled study. SETTING Andrology department at a university-based medical institution. PATIENT(S) Ten healthy volunteer sperm donors. INTERVENTION(S) Sperm populations were separated using annexin-V magnetic-activated ce...

In 3D domain swapping, a domain of a protein breaks its noncovalent bonds with the protein core and its place is taken by the identical domain of another molecule, creating a strongly bound dimer or higher order oligomer. For some proteins, including diphtheria toxin, 3D domain swapping may affect protein function. To explore the molecular basis of 3D domain swapping in a well-characterized pro...

OBJECTIVE To evaluate cryodamage on human sperm motility, cryosurvival rate, and sperm chromatin assessed by acridine orange staining method (AO test) after a six-month freeze-thawing process using liquid nitrogen vapor. STUDY DESIGN Experimental study. MATERIAL AND METHOD Twenty normal semen samples were obtained from the male partner of infertile couples attending the infertility unit, Si...

BACKGROUND Working toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the thawed specimens to remove concentration gradients resulting from freezing and thawing....

To ascertain a direct relationship between the light scattering intensity and the particle scattering the light, thin (6-20 micrometers) sections were cut from frozen human cataracts. Freezing for 15 min (-10 C) and then thawing had no effect on the clarity or light scattering properties of nuclear cataracts. Cortical cataracts treated in the same manner appeared to be much clearer than they we...

Several cryopreservation methods for precision-cut rat liver slices (PCLS) have been proposed, allowing a short-term (a few hours) maintainance of viability and functionality upon thawing. The aim of the present study was to test the metabolic capacity of PCLS cryopreserved by an ultrarapid method. The biotransformation of paracetamol to its glucuronide and sulfate conjugates and of midazolam t...

The purpose of the present study was to evaluate the effect of cryopreservation on 55 embryos which had one blastomere biopsied for preimplantation genetic diagnosis of aneuploidy before freezing. The thawing outcome was compared to that obtained in 94 embryos which derived from our conventional freezing programme in patients with comparable characteristics who were treated in the same period. ...

An enzymatic biotransformation process based on extracted pyruvate decarboxylase (PDC) overcomes the problem of by-product benzyl alcohol production. Seven methods of cells disruption which included application of glass beads, freezing/thawing sequence, silver nanoparticles, ultrasonication, freezing/thawing sequence with glass beads, freezing/thawing sequence with silver nanoparticles, and fre...