BACKGROUND Current prophylactic vaccines against human papillomavirus (HPV) target two of the most oncogenic types, HPV-16 and -18, which contribute to roughly 70% of cervical cancers worldwide. Second-generation HPV vaccines include a 9-valent vaccine, which targets five additional oncogenic HPV types (i.e., 31, 33, 45, 52, and 58) that contribute to another 15-30% of cervical cancer cases. Th...

Underwater volcano is an example of a natural habitat thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus this research was to screen the bacterial isolates originally from for production thermostable lipase. In study, lipase gene selected isolate cloned, sequenced, and silico analysis performed predict characteristics protein. One three examined, showed positive...

BACKGROUND Re-emergence of pertussis has been reported in Iran despite a high rate of vaccination coverage. Low efficacy of the vaccine might be due to the genetic divergence between clinical versus vaccine strains. In the current study, the genetic profiles of clinical isolates and vaccine strains of Bordetella pertussis (B. pertussis) were assessed by using Pulsed Field Gel Electrophoresis (P...

PART I GENETIC SELECTIONS 1 Genetic Complementation Protocols Jessica L. Sneeden and Lawrence A. Loeb ....................................... 3 2 Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase Manel Camps and Lawrence A. Loeb ................................................ 11 3 Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementa...

Spherical virus capsids are large, multimeric protein shells whose assembly and stability depend on the establishment of multiple non-covalent interactions between many polypeptide subunits. In a foot-and-mouth disease virus capsid, 42 amino acid side chains per protomer are involved in noncovalent interactions between pentameric subunits that function as assembly/disassembly intermediates. We ...

Unusual thermostable and insensitive high-energy compounds which contain dioximes with donor–acceptor H-bond sites, were synthesized from glyoxal (40% in water) straightforward steps.

A thermostable inhibition of ATP:protein phosphotransferase (EC 2.7.1.37) (protein kinase) which is present in crude tissue extracts has been resolved by gel chromatography (Sephadex G-100) into two molecular forms. These two forms will be referred to as type I and type II inhibitor. The type I inhibitor (M, = 24,000) is specific for CAMP-dependent protein kinase and corresponds to the inhibito...