Enhanced production of thermostable lipase from Bacillus cereus ASSCRC-P1 in waste frying oil based medium using statistical experimental designGhada E. A. Awad, Hanan Mostafa, Enas N. Danial, Nayera A.M. Abdelwahed, Hassan M. Awad

In this study, superparamagnetic iron oxide nanoparticles (MNPs) were synthesized via exposure of fungal cell filtrate from Aspergillus flavus to aqueous ions. The extracellular synthesis MNPs was monitored by UV–Vis spectrophotometry and showed an absorption peak at 310 nm. morphology found be flake-like, as confirmed Field Emission Scanning Electron Microscopy (FESEM), while the average cryst...

A simple procedure for the preparation of plant cell protoplasts is described. The leaf is first treated with pectinase under conditions that do not liberate cells; cellulase is then used to release protoplasts. The yields are comparable to those obtained by methods that entail the prior preparation of free cells. The method is of general application but is particularly useful with tissues that...

The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evo...

The 14.4-kDa hexon-associated protein IX (pIX) acts as a cement in the capsids of primate adenoviruses and confers a thermostable phenotype. Here we show that deletion of amino acids 100 to 114 of adenovirus type 5 pIX, which eliminates the conserved coiled-coil domain, impairs its capacity to self-associate. However, pIXDelta100-114 is efficiently incorporated into the viral capsid, and the re...

Virulence factor genes encoding the thermostable direct hemolysin (tdh) and the thermostable direct hemolysin-related hemolysin (trh) are strongly correlated with virulence of the emergent human pathogen Vibrio parahaemolyticus. The gene encoding the thermolabile hemolysin (tlh) is also considered a signature molecular marker for the species. These genes are typically reported in very low perce...

Vibrio parahaemolyticus has emerged as a major foodborne pathogen in China, Japan, Thailand, and other Asian countries. In this study, 72 strains of V. parahaemolyticus were isolated from clinical and environmental samples between 2006 and 2014 in Jiangsu, China. The serotypes and six virulence genes including thermostable direct hemolysin (TDR) and TDR-related hemolysin (TRH) genes were assess...

Banana besides its nutritive value the biomass can be used as resources for natural fibre. North east India and Assam in particular has a vast potential of extraction and value addition of natural fibre, especially from banana. Banana farming generates huge quantities of biomass most of which goes as waste due to non‐availability of suitable technology for its commercial utilization. Banana fib...