The enzyme rhodanese (EC 2.8.1.1) could be reversibly refolded from urea in the presence of lauryl maltoside, beta-mercaptoethanol, and sodium thiosulfate. The unfolding/folding transition monitored using intrinsic fluorescence was resolved into two two-state transitions with midpoints at 3.6 and 5.0 M urea. The analysis assumed an intermediate with an emission maximum at 345 nm. Monitoring ani...

Production of sulfur globules during sulfide or thiosulfate oxidation is a characteristic feature of some sulfur bacteria. Although their generation has been reported in Acidithiobacillus ferrooxidans, its mechanism of formation and deposition, as well as the physiological significance of these globules during sulfur compounds oxidation, are currently unknown. Under oxygen-sufficient conditions...

Thiobacillus novellus was able to grow with oxalate, formate, formamide, and methanol as sole sources of carbon and energy. Extensive growth on methanol required yeast extract or vitamins. Glyoxylate carboligase was detected in extracts of oxalate-grown cells. Ribulose bisphosphate carboxylase was found in extracts of cells grown on formate, formamide, and thiosulfate. These data indicate that ...

Several members of the order Thermotogales in the domain Bacteria, viz., Thermotoga neapolitana, Thermotoga maritima, Thermosipho africanus, Fervidobacterium islandicum, and Thermotoga strain SEBR 2665, an isolate from an oil well, reduced thiosulfate to sulfide. This reductive process enhanced cellular yields and growth rates of all the members but was more significant with the two hyperthermo...

The kinetics of adsorption of self-assembled monolayers (SAMs) from solutions of hexadecyl thiosulfate in tetrahydrofuran was studied under a variety of experimental conditions to elucidate the mechanism(s) important in this process. Monolayers did not form in the absence of water, which ruled out the direct addition of the alkyl thiosulfate to the gold surface and implicated hydrolysis as a ro...

Thiosulfate is an inorganic ligand that forms a soluble light-sensitive cadmium thiosulfate complex, and sulfide (CdS) with “size effect” can be produced via ultraviolet (UV) irradiation. This study investigated the activity of CdS nanoparticles (NPs) precipitated from complex UV irradiation on photocatalytic dye degeneration. Scanning electron microscopy (SEM) transmission (TEM) demonstrated d...