This study evaluated the human pulp tissue response following direct capping with Clearfil Protect Bond (CPB) self-etching adhesive containing an antibacterial monomer MDPB. The pulps of third molar teeth were exposed by removal carious tissue. In experimental group, CPB was applied to and dentin. control groups, SE (CSE) or calcium hydroxide-based cement (CH) surfaces. All filled resin composi...

With the increased use of gene expression profiling to identify molecular regulators of cellular and developmental mechanisms, developmental biologists face a new challenge in dissecting tissues without cross-contamination or change in RNA profile, and with intact RNA integrity. We have developed a technique that overcomes these problems. We took the dissection of rudimentary mouse embryonic ma...

The evidence that exposure to polychlorinated biphenyls (PCBs) leads to mutations is equivocal and controversial. Using multilocus DNA fingerprinting, we compared the mutation rate of tree swallows (Tachycineta bicolor) nesting at sites with high and low levels of contamination with PCBs. The upper Hudson River, USA, is highly contaminated with PCBs as a result of releases from two capacitor ma...

Pleuropneumonia-like organisms (PPLO) are the crabgrass of tissue culture. Many reports exist in the literature of successful attempts to eliminate these organisms from tissue cultures, but usually such reports were stimulated by the failure of some previously described method to be successful in the hands of the investigator. Although our cultures exhibited no overt manifestations of contamina...

Trimming procedures are an important part of the sequence analysis pipeline in an EST Sequencing Project. In general, trimming is done in several phases, each one detecting and removing some kind of undesirable artifact, such as low quality sequence, vectors or adapters sequence, and contamination. However, this strategy often results in a phase being unable to recognize its target because part...

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

Abstract Sample cross-contamination remains a pervasive issue in genetics and genomics. With growing reliance on molecular methods for managing marine resources, the need to ensure integrity of tissue samples that underpin these has never been more pressing. We conducted an experiment wild-caught bigeye tuna (Thunnus obesus) assess risk under seven at-sea laboratory-based sampling treatments. T...

Anthropogenic environmental changes are hypothesized as main reasons for animal species population declines. Heavy metals contamination is one of the worst threats to animals among human-caused threats. As most of the heavy metals bioaccumulate in organisms, analyzing concentrations of heavy metals in long living animals, such as turtles, would be very useful for biomonitoring of environmental ...