نتایج جستجو برای: tlypanosoma rangeli
تعداد نتایج: 239 فیلتر نتایج به سال:
The isolation of biological clones of Trypanosoma cruzi by microscopically dispensing individual organisms or by serial dilution is laborious and time consuming. The inability to resolve mixed T. cruzi infections, from vectors and hosts, and to isolate clones of slow growing genotypes by efficient plating on solid media, has hindered characterisation studies and downstream applications. We have...
A DNA-DNA hybridization procedure is described in which radiolabeled total parasite DNA is used to detect Trypanosoma cruzi spotted directly onto nylon membrane. Under conditions that favor hybridization of repetitive DNA sequences, the radiolabeled total DNA was able to detect as few as five parasites spotted onto nylon membrane. Trypanosomes were detectable in blood from mice with T. cruzi pa...
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