The reporter strain Pseudomonas putida TOD102 (with a tod-lux fusion) was used in chemostat experiments with binary substrate mixtures to investigate the effect of potentially occurring cosubstrates on toluene degradation activity. Although toluene was simultaneously utilized with other cosubstrates, its metabolic flux (defined as the toluene utilization rate per cell) decreased with increasing...

Previously, Planted Fixed-Bed Reactors (PFRs) have been used to investigate microbial toluene removal in the rhizosphere of constructed wetlands. Aerobic toluene degradation was predominant in these model systems although bulk redox conditions were hypoxic to anoxic. However, culture-independent approaches indicated also that microbes capable of anaerobic toluene degradation were abundant. Ther...

The title compound, [Mn(C(18)H(18)N(2)O(4))(C(7)H(5)O(3))(H(2)O)], was synthesized by a template reaction of ethane-1,2-diamine and 3-methoxy-salicylaldehyde in presence of manganese(II) 4-hydroxy-benzoate. The Jahn-Teller-distorted manganese(III) centre has an octa-hedral geometry. Extensive O-H⋯O hydrogen-bonding inter-actions generate a two-dimensional sheet structure parallel to (103).

Workers exposed to toluene develop many central and autonomic nervous symptoms. It has been suggested that the effects of toluene on the central nervous system may be detectable by EEG. In the present experiments, changes in EEG and behaviour of rats exposed to toluene were monitored in an attempt to clarify the relationship between exposure to toluene and central nervous system reaction. Chron...

Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in pe...

Transfer ribonucleic acid (tRNA) nucleotidyltransferase was studied after making cells permeable to macromolecules by treatment with toluene. The conditions of toluene treatment necessary for obtaining maximal activity were defined. Toluene treatment was most efficient when carried out for 5 min at 37 C at pH 9.0 on log-phase cells. No activity could be detected if cells were treated at 0 C, or...

Using toluene droplets as a model for artificial oxygen carriers, the real-time measurement of attomole oxygen contents at the individual droplet level is reported for the first time. Oxygen reduction is observed within single individual toluene droplets when they impact with an electrode held at a suitable potential. The oxygen content within single toluene droplets is quantified by referencin...

Permeabilized cells of toluene-mineralizing, sulfate-reducing strain PRTOL1 catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. Recent in vitro studies with two toluene-mineralizing, denitrifying bacteria demonstrated the same fumarate addition reaction and indicated that it may be the first step of anaerobic toluene degradation. This study with str...